D-lactic acid biosynthesis from biomass-derived sugars via Lactobacillus delbrueckii fermentation
dc.citation.doi | doi:10.1007/s00449-013-0965-8 | en_US |
dc.citation.epage | 1904 | en_US |
dc.citation.issue | 12 | en_US |
dc.citation.jtitle | Bioprocess and Biosystems Engineering | en_US |
dc.citation.spage | 1897 | en_US |
dc.citation.volume | 36 | en_US |
dc.contributor.author | Zhang, Yixing | |
dc.contributor.author | Vadlani, Praveen V. | |
dc.contributor.authoreid | vadlani | en_US |
dc.date.accessioned | 2014-01-22T21:08:21Z | |
dc.date.available | 2014-01-22T21:08:21Z | |
dc.date.issued | 2014-01-22 | |
dc.date.published | 2013 | en_US |
dc.description.abstract | Poly-lactic acid (PLA) derived from renewable resources is considered to be a good substitute for petroleum-based plastics. The number of poly L-lactic acid applications is increased by the introduction of a stereocomplex PLA, which consists of both poly-L and D-lactic acid and has a higher melting temperature. To date, several studies have explored the production of L-lactic acid, but information on biosynthesis of D-lactic acid is limited. Pulp and corn stover are abundant, renewable lignocellulosic materials that can be hydrolyzed to sugars and used in biosynthesis of D-lactic acid. In our study, saccharification of pulp and corn stover was done by cellulase CTec2 and sugars generated from hydrolysis were converted to D-lactic acid by a homofermentative strain, L. delbrueckii, through a sequential hydrolysis and fermentation process (SHF) and a simultaneous saccharification and fermentation process (SSF). 36.3 g Lˉ¹ of D-lactic acid with 99.8 % optical purity was obtained in the batch fermentation of pulp and attained highest yield and productivity of 0.83 g gˉ¹ and 1.01 g Lˉ¹ hˉ¹, respectively. Luedeking–Piret model described the mixed growth-associated production of D-lactic acid with a maximum specific growth rate 0.2 hˉ¹ and product formation rate 0.026 hˉ¹, obtained for this strain. The efficient synthesis of D-lactic acid having high optical purity and melting point will lead to unique stereocomplex PLA with innovative applications in polymer industry. | en_US |
dc.identifier.uri | http://hdl.handle.net/2097/17051 | |
dc.language.iso | en_US | en_US |
dc.relation.uri | http://link.springer.com/article/10.1007/s00449-013-0965-8 | en_US |
dc.rights | The final publication is available at link.springer.com. | en_US |
dc.subject | D-lactic acid | en_US |
dc.subject | Fermentation | en_US |
dc.subject | Corn stover | en_US |
dc.subject | Pulp | en_US |
dc.subject | Biosynthesis | en_US |
dc.title | D-lactic acid biosynthesis from biomass-derived sugars via Lactobacillus delbrueckii fermentation | en_US |
dc.type | Article (author version) | en_US |