Lipidomic Profiling of Di- and Tri-Acylglycerol Species in Weight-Controlled Mice

dc.citation.doi10.1371/journal.pone.0116398
dc.citation.issn1932-6203
dc.citation.issue2
dc.citation.jtitlePLoS One
dc.citation.spage12
dc.citation.volume10
dc.contributor.authorKing, B. S.
dc.contributor.authorLu, L. Z.
dc.contributor.authorYu, M.
dc.contributor.authorJiang, Yu
dc.contributor.authorStandard, J.
dc.contributor.authorSu, X. Y.
dc.contributor.authorZhao, Z. H.
dc.contributor.authorWang, Weiqun
dc.contributor.authoreidwwang
dc.date.accessioned2016-04-06T14:51:32Z
dc.date.available2016-04-06T14:51:32Z
dc.date.issued2015-02-23
dc.date.issued2015-02-23
dc.date.published2015
dc.descriptionCitation: King, B. S., Lu, L. Z., Yu, M., Jiang, Y., Standard, J., Su, X. Y., . . . Wang, W. Q. (2015). Lipidomic Profiling of Di- and Tri-Acylglycerol Species in Weight-Controlled Mice. Plos One, 10(2), 12. doi:10.1371/journal.pone.0116398
dc.descriptionWeight control by dietary calorie restriction (DCR) or exercise has been shown to prevent cancer in various models. However, the mechanisms as to how weight control is beneficial are not well understood. While previous reports have investigated the effects of weight control on total lipid levels or lipid composition within cellular membranes, there has been little work surrounding changes to individual lipids following weight control interventions. In this study, using a model of skin carcinogenesis centered on the tumor promotion stage, CD-1 mice were randomly assigned into 4 groups: ad libitum and sedentary (control), ad libitum with exercise (AL+Exe), exercise with pair feeding of a diet isocaloric with control (PF+Exe), and sedentary with 20% DCR compared to control. After ten weeks, body weight and body fat percentages significantly decreased in the PF+Exe and DCR groups but not AL+Exe when compared with sedentary controls. Murine skin and plasma samples were obtained for analysis. Lipidomics using electrospray ionization MS/MS was employed to profile triacylglycerol (TG) and diacylglycerol (DG) species. Both plasma and tissue TG species containing fatty acid chains with length 18: 1 were significantly decreased following DCR when compared to sedentary control animals. In regards to DG, the most significant changes occurred in the plasma. DG species containing fatty acids with lengths 16: 1 or 18: 1 were significantly decreased in PF+Exe and DCR groups when compared to sedentary controls. Due to the significant role of TG in energy storage and DG in cellular signaling, our findings of the effects of weight control on individual TG and DG species in plasma and skin tissue following exposure to a tumor promoter, may provide insight into the mechanism of weight control on cancer prevention.
dc.description.versionArticle: Version of Record
dc.identifier.urihttp://hdl.handle.net/2097/32331
dc.relation.urihttps://doi.org/10.1371/journal.pone.0116398
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectProtein-Kinase-C
dc.subjectCalorie-Restricted Mice
dc.subjectFatty-Acid-Composition
dc.subjectCancer Prevention
dc.subjectMass-Spectrometry
dc.subjectIn-Vitro
dc.titleLipidomic Profiling of Di- and Tri-Acylglycerol Species in Weight-Controlled Mice
dc.typeText

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