Inward-rectifier chloride currents in Reissner’s membrane epithelial cells

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Show simple item record Kim, Kyunghee 2010-05-03T17:24:51Z 2010-05-03T17:24:51Z 2010-05-03T17:24:51Z
dc.description.abstract Sensory transduction in the cochlea depends on regulated ion secretion and absorption. Results of whole-organ experiments suggested that Reissner’s membrane may play a role in the control of luminal Cl-. We tested for the presence of Cl- transport pathways in isolated mouse Reissner’s membrane using whole-cell patch clamp recordings and gene transcript analyses using RT-PCR. The current-voltage (I-V) relationship in the presence of symmetrical NMDG-Cl was strongly inward-rectifying at negative voltages, with a small outward current at positive voltages. The inward-rectifying component of the I-V curve had several properties similar to those of the ClC-2 Cl- channel. It was stimulated by extracellular acidity and inhibited by extracellular Cd2+, Zn2+, and intracellular ClC-2 antibody. Channel transcripts expressed in Reissner’s membrane include ClC-2, Slc26a7 and ClC-Ka, but not Cftr, ClC-1, ClCa1, ClCa2, ClCa3, ClCa4, Slc26a9, ClC-Kb, Best1, Best2, Best3 or the beta-subunit of ClC-K, barttin. ClC-2 is the only molecularly-identified channel present that is a strong inward rectifier. This thesis incorporates the publication by K.X. Kim and D.C. Marcus, Inward-rectifier chloride currents in Reissner’s membrane epithelial cells. Biochem. Biophys. Res. Commun. 394 (2010) 434-438, with permission of the publisher Elsevier, and is the first report of conductive Cl- transport in epithelial cells of Reissner’s membrane and is consistent with an important role in endolymph anion homeostasis. en_US
dc.description.sponsorship This work was supported by grants to Daniel C. Marcus from the National Institutes of Health R01-DC00212 and P20-RR017686. en_US
dc.language.iso en_US en_US
dc.publisher Kansas State University en
dc.subject Cl- channel en_US
dc.subject Epithelial transport en_US
dc.subject Cochlea en_US
dc.subject ClC-2 channel en_US
dc.title Inward-rectifier chloride currents in Reissner’s membrane epithelial cells en_US
dc.type Thesis en_US Master of Science en_US
dc.description.level Masters en_US
dc.description.department Department of Anatomy and Physiology en_US
dc.description.advisor Daniel C. Marcus en_US
dc.subject.umi Biology, Veterinary Science (0778) en_US 2010 en_US May en_US

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