Inward-rectifier chloride currents in Reissner’s membrane epithelial cells
dc.contributor.author | Kim, Kyunghee | |
dc.date.accessioned | 2010-05-03T17:24:51Z | |
dc.date.available | 2010-05-03T17:24:51Z | |
dc.date.graduationmonth | May | en_US |
dc.date.issued | 2010-05-03T17:24:51Z | |
dc.date.published | 2010 | en_US |
dc.description.abstract | Sensory transduction in the cochlea depends on regulated ion secretion and absorption. Results of whole-organ experiments suggested that Reissner’s membrane may play a role in the control of luminal Cl-. We tested for the presence of Cl- transport pathways in isolated mouse Reissner’s membrane using whole-cell patch clamp recordings and gene transcript analyses using RT-PCR. The current-voltage (I-V) relationship in the presence of symmetrical NMDG-Cl was strongly inward-rectifying at negative voltages, with a small outward current at positive voltages. The inward-rectifying component of the I-V curve had several properties similar to those of the ClC-2 Cl- channel. It was stimulated by extracellular acidity and inhibited by extracellular Cd2+, Zn2+, and intracellular ClC-2 antibody. Channel transcripts expressed in Reissner’s membrane include ClC-2, Slc26a7 and ClC-Ka, but not Cftr, ClC-1, ClCa1, ClCa2, ClCa3, ClCa4, Slc26a9, ClC-Kb, Best1, Best2, Best3 or the beta-subunit of ClC-K, barttin. ClC-2 is the only molecularly-identified channel present that is a strong inward rectifier. This thesis incorporates the publication by K.X. Kim and D.C. Marcus, Inward-rectifier chloride currents in Reissner’s membrane epithelial cells. Biochem. Biophys. Res. Commun. 394 (2010) 434-438, with permission of the publisher Elsevier, and is the first report of conductive Cl- transport in epithelial cells of Reissner’s membrane and is consistent with an important role in endolymph anion homeostasis. | en_US |
dc.description.advisor | Daniel C. Marcus | en_US |
dc.description.degree | Master of Science | en_US |
dc.description.department | Department of Anatomy and Physiology | en_US |
dc.description.level | Masters | en_US |
dc.description.sponsorship | This work was supported by grants to Daniel C. Marcus from the National Institutes of Health R01-DC00212 and P20-RR017686. | en_US |
dc.identifier.uri | http://hdl.handle.net/2097/3866 | |
dc.language.iso | en_US | en_US |
dc.publisher | Kansas State University | en |
dc.subject | Cl- channel | en_US |
dc.subject | Epithelial transport | en_US |
dc.subject | Cochlea | en_US |
dc.subject | ClC-2 channel | en_US |
dc.subject.umi | Biology, Veterinary Science (0778) | en_US |
dc.title | Inward-rectifier chloride currents in Reissner’s membrane epithelial cells | en_US |
dc.type | Thesis | en_US |