Characterization of Fusobacterium isolates from the respiratory tract of white-tailed deer (Odocoileus virginianus)

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Show simple item record Brooks, Jason W. Kumar, Amit Narayanan, Sanjeev K. Myers, Suzanne Brown, Kayla Nagaraja, Tiruvoor G. Jayarao, Bhushan M. 2014-07-22T14:40:53Z 2014-07-22T14:40:53Z 2014-03-03
dc.description.abstract A total of 23 clinical isolates of Fusobacterium spp. were recovered at necropsy over a 2-year period from the respiratory tract of white-tailed deer (Odocoileus virginianus). Isolates were identified as Fusobacterium varium (18/23), Fusobacterium necrophorum subsp. funduliforme (3/23), and Fusobacterium necrophorum subsp. necrophorum (2/23). Using polymerase chain reaction–based detection of virulence genes, all F. necrophorum isolates were positive for the promoter region of the leukotoxin operon and the hemagglutinin-related protein gene, while all F. varium isolates were negative. The presence of the leukotoxin gene in F. necrophorum isolates and the absence of this gene in F. varium isolates were confirmed by Southern hybridization using 2 separate probes. Toxicity to bovine polymorphonuclear leukocytes was observed with all F. necrophorum isolates, but was not observed in any F. varium isolates. Susceptibility to antimicrobials was markedly different for F. varium as compared to F. necrophorum. In summary, no evidence of leukotoxin production was detected in any of the 23 F. varium isolates used in the current study. The data suggests that F. varium, the most common species isolated, may be a significant pathogen in deer with a different virulence mechanism than F. necrophorum. en_US
dc.language.iso en_US en_US
dc.relation.uri en_US
dc.subject Fusobacterium necrophorum en_US
dc.subject Fusobacterium varium en_US
dc.subject Leukotoxin en_US
dc.subject White-tailed deer en_US
dc.title Characterization of Fusobacterium isolates from the respiratory tract of white-tailed deer (Odocoileus virginianus) en_US
dc.type Article (author version) en_US 2014 en_US
dc.citation.doi 10.1177/1040638714523613 en_US
dc.citation.epage 220 en_US
dc.citation.issue 2 en_US
dc.citation.jtitle Journal of Veterinary Diagnostic Investigation en_US
dc.citation.spage 213 en_US
dc.citation.volume 26 en_US
dc.contributor.authoreid amitk en_US
dc.contributor.authoreid snarayan en_US
dc.contributor.authoreid tnagaraj en_US

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