Characterization of Fusobacterium isolates from the respiratory tract of white-tailed deer (Odocoileus virginianus)

dc.citation.doi10.1177/1040638714523613en_US
dc.citation.epage220en_US
dc.citation.issue2en_US
dc.citation.jtitleJournal of Veterinary Diagnostic Investigationen_US
dc.citation.spage213en_US
dc.citation.volume26en_US
dc.contributor.authorBrooks, Jason W.
dc.contributor.authorKumar, Amit
dc.contributor.authorNarayanan, Sanjeev K.
dc.contributor.authorMyers, Suzanne
dc.contributor.authorBrown, Kayla
dc.contributor.authorNagaraja, Tiruvoor G.
dc.contributor.authorJayarao, Bhushan M.
dc.contributor.authoreidamitken_US
dc.contributor.authoreidsnarayanen_US
dc.contributor.authoreidtnagarajen_US
dc.date.accessioned2014-07-22T14:40:53Z
dc.date.available2014-07-22T14:40:53Z
dc.date.issued2014-03-03
dc.date.published2014en_US
dc.description.abstractA total of 23 clinical isolates of Fusobacterium spp. were recovered at necropsy over a 2-year period from the respiratory tract of white-tailed deer (Odocoileus virginianus). Isolates were identified as Fusobacterium varium (18/23), Fusobacterium necrophorum subsp. funduliforme (3/23), and Fusobacterium necrophorum subsp. necrophorum (2/23). Using polymerase chain reaction–based detection of virulence genes, all F. necrophorum isolates were positive for the promoter region of the leukotoxin operon and the hemagglutinin-related protein gene, while all F. varium isolates were negative. The presence of the leukotoxin gene in F. necrophorum isolates and the absence of this gene in F. varium isolates were confirmed by Southern hybridization using 2 separate probes. Toxicity to bovine polymorphonuclear leukocytes was observed with all F. necrophorum isolates, but was not observed in any F. varium isolates. Susceptibility to antimicrobials was markedly different for F. varium as compared to F. necrophorum. In summary, no evidence of leukotoxin production was detected in any of the 23 F. varium isolates used in the current study. The data suggests that F. varium, the most common species isolated, may be a significant pathogen in deer with a different virulence mechanism than F. necrophorum.en_US
dc.identifier.urihttp://hdl.handle.net/2097/18125
dc.language.isoen_USen_US
dc.relation.urihttp://doi.org/10.1177/1040638714523613en_US
dc.subjectFusobacterium necrophorumen_US
dc.subjectFusobacterium variumen_US
dc.subjectLeukotoxinen_US
dc.subjectWhite-tailed deeren_US
dc.titleCharacterization of Fusobacterium isolates from the respiratory tract of white-tailed deer (Odocoileus virginianus)en_US
dc.typeArticle (author version)en_US

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