Combination of PB2 271A and SR polymorphism at positions 590/591 is critical for viral replication and virulence of swine influenza virus in cultured cells and in vivo
dc.citation.doi | 10.1128/JVI.05699-11 | en_US |
dc.citation.epage | 1237 | en_US |
dc.citation.issue | 2 | en_US |
dc.citation.jtitle | Journal of Virology | en_US |
dc.citation.spage | 1233 | en_US |
dc.citation.volume | 86 | en_US |
dc.contributor.author | Liu, Qinfang | |
dc.contributor.author | Qiao, Chuanling | |
dc.contributor.author | Marjuki, Henju | |
dc.contributor.author | Bawa, Bhupinder | |
dc.contributor.author | Ma, Jingqun | |
dc.contributor.author | Guillossou, Stephane | |
dc.contributor.author | Webby, Richard J. | |
dc.contributor.author | Richt, Juergen A. | |
dc.contributor.author | Ma, Wenjun | |
dc.contributor.authoreid | jricht | en_US |
dc.contributor.authoreid | bbawa | en_US |
dc.contributor.authoreid | mjq0819 | en_US |
dc.contributor.authoreid | sguill | en_US |
dc.contributor.authoreid | wjma | en_US |
dc.date.accessioned | 2012-06-27T19:18:27Z | |
dc.date.available | 2012-06-27T19:18:27Z | |
dc.date.issued | 2011-12-28 | |
dc.date.published | 2012 | en_US |
dc.description.abstract | Triple reassortant swine influenza viruses (SIVs) and 2009 pandemic H1N1 (pH1N1) virus contain an avian-origin PB2 with 271A, 590S, 591R, and 627E. To evaluate the role of PB2 271A, 590S, and 591R in the replication and virulence of SIV, single (1930-TX98-PB2-271T)-, double (1930-TX98-PB2-590A591A)-, and triple (1930-TX98-PB2-271T590A591A)-mutated viruses were generated in the background of the H1N1 A/swine/Iowa/15/30 (1930) virus with an avian-origin PB2 from the triple-reassortant A/swine/Texas/4199-2/98 (TX98) virus, called the parental 1930-TX98-PB2. Compared to parental virus and single- and double-mutated viruses, the triple-mutated virus replicated less efficiently in cell cultures and was attenuated in mice. These results suggest that a combination of 271A with the 590/591 SR polymorphism is critical for pH1N1 and triple-reassortant SIVs for efficient replication and adaptation in mammals. | en_US |
dc.identifier.uri | http://hdl.handle.net/2097/13965 | |
dc.relation.uri | http://doi.org/10.1128/JVI.05699-11 | en_US |
dc.subject | Swine influenza virus | en_US |
dc.subject | PB2 | en_US |
dc.subject | 271A | en_US |
dc.subject | 590/591 SR polymorphism | en_US |
dc.subject | Replication and virulence | en_US |
dc.subject | Cultured cells | en_US |
dc.subject | In vivo | en_US |
dc.title | Combination of PB2 271A and SR polymorphism at positions 590/591 is critical for viral replication and virulence of swine influenza virus in cultured cells and in vivo | en_US |
dc.type | Article (author version) | en_US |
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