Detection of Escherichia coli O104 in the feces of feedlot cattle by a multiplex PCR assay designed to target major genetic traits of the virulent hybrid strain responsible for the 2011 German outbreak

Paddock, Zachary Dean; Bai, Jianfa; Shi, X.; Renter, David G.; Nagaraja, Tiruvoor G.

Detection of Escherichia coli O104 in the feces of feedlot cattle by a multiplex PCR assay designed to target major genetic traits of the virulent hybrid strain responsible for the 2011 German outbreak

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NagarajaApplEnviroMicrobio2013.pdf (196.15 KB)

Appl. Environ. Microbiol.-2013-Paddock-3522-5.pdf (304.13 KB)

Date

2013-05-06, 2013-06-01

Authors

Paddock, Zachary Dean

Bai, Jianfa

Shi, X.

Renter, David G.

Nagaraja, Tiruvoor G.

Abstract

A multiplex PCR was designed to detect E. coli O104:H4, a hybrid pathotype of Shiga toxigenic and enteroaggregative E. coli, in cattle feces. A total of 248 fecal samples were tested and 20.6% were positive for the O104 serogroup. The isolates of O104 did not carry genes characteristic of the virulent hybrid strain.