Analysis of Acquisition and Titer of Maize Mosaic Rhabdovirus in Its Vector, Peregrinus maidis (Hemiptera: Delphacidae)

dc.citationBarandoc-Alviar, K., Ramirez, G. M., Rotenberg, D., & Whitfield, A. E. (2016). Analysis of Acquisition and Titer of Maize Mosaic Rhabdovirus in Its Vector, Peregrinus maidis (Hemiptera: Delphacidae). Journal of Insect Science, 16(1), 1-8. https://doi.org/10.1093/jisesa/iev154
dc.citation.doi10.1093/jisesa/iev154
dc.citation.issn1536-2442
dc.citation.jtitleJournal of Insect Science
dc.citation.spage8
dc.citation.volume16
dc.contributor.authorBarandoc-Alviar, K.
dc.contributor.authorRamirez, G. M.
dc.contributor.authorRotenberg, Dorith
dc.contributor.authorWhitfield, Anna E.
dc.contributor.authoreiddrotenbe
dc.contributor.authoreidaewtospo
dc.date.accessioned2016-09-20T16:54:52Z
dc.date.available2016-09-20T16:54:52Z
dc.date.issued2016-02-06
dc.date.published2016
dc.descriptionCitation: Barandoc-Alviar, K., Ramirez, G. M., Rotenberg, D., & Whitfield, A. E. (2016). Analysis of Acquisition and Titer of Maize Mosaic Rhabdovirus in Its Vector, Peregrinus maidis (Hemiptera: Delphacidae). Journal of Insect Science, 16(1), 1-8. https://doi.org/10.1093/jisesa/iev154
dc.description.abstractThe corn planthopper, Peregrinus maidis (Ashmead) (Hemiptera: Delphacidae), transmits Maize mosaic rhabdovirus (MMV), an important pathogen of maize and sorghum, in a persistent propagative manner. To better understand the vectorial capacity of P. maidis, we determined the efficiency of MMV acquisition by nymphal and adult stages, and characterized MMV titer through development. Acquisition efficiency, i.e., proportion of insects that acquired the virus, was determined by reverse transcriptase polymerase chain reaction (RT-PCR) and virus titer of individual insects was estimated by quantitative RT-PCR. Acquisition efficiency of MMV differed significantly between nymphs and adults. MMV titer increased significantly over time and throughout insect development from nymphal to adult stage, indication of virus replication in the vector during development. There was a positive association between the vector developmental stage and virus titer. Also, the average titer in male insects was threefold higher than female titers, and this difference persisted up to 30 d post adult eclosion. Overall, our findings indicate that nymphs are more efficient than adults at acquiring MMV and virus accumulated in the vector over the course of nymphal development. Furthermore, sustained infection over the lifespan of P. maidis indicates a potentially high capacity of this vector to transmit MMV.
dc.description.versionArticle: Version of Record
dc.identifier.urihttp://hdl.handle.net/2097/33967
dc.relation.urihttps://doi.org/10.1093/jisesa/iev154
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.urihttps://academic.oup.com/journals/pages/self_archiving_policy_c
dc.subjectVector-Virus Interaction
dc.subjectPropagative Transmission
dc.subjectAcquisition
dc.subjectEfficiency
dc.subjectSustained Infection
dc.subjectSpotted Wilt Tospovirus
dc.titleAnalysis of Acquisition and Titer of Maize Mosaic Rhabdovirus in Its Vector, Peregrinus maidis (Hemiptera: Delphacidae)
dc.typeText

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