Use of recombinant bovine cytokines in pigs vaccinated and challenged with streptococcus suis

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Show simple item record Blecha, Frank Reddy, D.N. Chitko-McKown, C.G. Chengappa, M. M. McVey, D.S. Goodband, Robert D. Nelssen, Jim L. 2010-04-08T19:59:43Z 2010-04-08T19:59:43Z 2010-04-08T19:59:43Z
dc.description.abstract An experiment was conducted to determine the adjuvanticity of recombinant bovine interleukin-1β (rBoIL-1β) and recombinant bovine interleukin-2 (rBoIL-2) administered in conjunction with a single S. suis vaccination in pigs. Sixty, 4-wk-old pigs were allotted to 8 groups: 1) nonvaccinated controls; 2) vaccinated controls; 3) rBoIL-Iβ, 100 ng/kg; 4)rBoIL-lβ, 1000 ng/kg; 5) rBoIL-1β, 10,000 ng/kg; 6) rBoIL-2, 2.5 µg/kg; 7) rBoIL-2, 25 µg/kg; and 8) rBoIL-2, 250 µg/kg. All pigs (except group 1) were vaccinated on d 0 with a commercial S. suis vaccine (serotypes 1 and 2). At vaccination, pigs were injected intramuscularly with their respective cytokine treatments. Pigs received additional cytokine injections for 2 consecutive days. On d 21, all pigs were injected intravenously with 3.5 x 109 CFU of a log phase culture of S. suis (serotype 2). The highest dose of rBolL-1β exceeded the maximum tolerable dose for the cytokine; however, this dose of rBoIL-1β protected pigs from the S. suis challenge. In pigs receiving rBoIL-1β at 10,000 ng/kg, pathological lesions caused by S. suis were lowest when compared to other treatment groups. No mortality from S. suis challenge was observed in pigs that received the highest dose of rBoIL-1β. These data clearly show that rBoIL-1β (10,000 ng/kg), administered intramuscularly for 3 consecutive days at vaccination, is more effective than the S. suis vaccine alone in protecting pigs against a S. suis challenge. en_US
dc.publisher Kansas State University. Agricultural Experiment Station and Cooperative Extension Service en_US
dc.relation.isPartOf Swine day, 1991 en_US
dc.relation.isPartOf Kansas Agricultural Experiment Station contribution; no. 92-193-S en_US
dc.relation.isPartOf Report of progress (Kansas State University. Agricultural Experiment Station and Cooperative Extension Service); 641 en_US
dc.subject Swine en_US
dc.subject Cytokine en_US
dc.subject Adjuvant en_US
dc.subject Pig en_US
dc.subject Vaccine en_US
dc.title Use of recombinant bovine cytokines in pigs vaccinated and challenged with streptococcus suis en_US
dc.type Conference paper en_US 1991 en_US
dc.citation.epage 140 en_US
dc.citation.spage 137 en_US
dc.description.conference Swine Day, Manhattan, KS, November 21, 1991 en_US
dc.contributor.authoreid blecha en_US
dc.contributor.authoreid chengapa en_US
dc.contributor.authoreid goodband en_US
dc.contributor.authoreid jnelssen en_US

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