Temporal changes in the bacterial community of animal feces and their correlation with stable fly oviposition, larval development, and adult fitness

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dc.contributor.author Albuquerque, Thais Aguiar De
dc.contributor.author Zurek, Ludek
dc.date.accessioned 2015-05-13T18:53:54Z
dc.date.available 2015-05-13T18:53:54Z
dc.date.issued 2015-05-13
dc.date.issued 2014-11-10
dc.identifier.uri http://hdl.handle.net/2097/19243
dc.description.abstract Stable flies are blood-feeding insects with a great negative impact on animals world wide. Larvae develop primarily in animal manure and bacteria are essential for larval development; however, the principle of this dependence is not understood. We hypothesized that as the microbial community of animal manure changes over time, it plays an important role in stable fly fitness. Two-choice bioassays were conducted using 2 week old horse manure (control) and aging horse manure (fresh to 5 week old) to evaluate the effect of manure age on stable fly oviposition. Our data showed that fresh feces did not stimulate oviposition and that the attractiveness increased as manure aged but started to decline after 3 weeks. Bioassays assessing the effect of manure age at the time of oviposition on larval development demonstrated that 1–3 week old manure supported larval development significantly better than fresh, 4, and 5 week old manure. In addition, adult fitness (body size) was significantly higher in flies from 1 and 2 week old manure comparing to that of all other treatments. Analysis of the bacterial community of aging horse manure by 454-pyrosequencing of 16S rDNA revealed a great reduction in bacterial diversity and richness from fresh to 1–5 week old manure and a major shift from strict anaerobes in fresh manure to facultative anaerobes and strict aerobes in aged manure. Overall, the microbial community of 2 and 3 week old horse manure with its dominant bacterial taxa Rhizobium, Devosia, and Brevundimonas stimulated stable fly oviposition the most and provided a suitable habitat for larval development. These bacteria represent the candidates for studies focused on better understanding of stable fly – microbial interactions. en_US
dc.language.iso en_US en_US
dc.relation.uri http://journal.frontiersin.org/article/10.3389/fmicb.2014.00590/abstract en_US
dc.rights Attribution 4.0 International (CC BY 4.0) en_US
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en_US
dc.subject Stable fly en_US
dc.subject Oviposition en_US
dc.subject Horse feces en_US
dc.subject Bacteria en_US
dc.subject Diversity en_US
dc.title Temporal changes in the bacterial community of animal feces and their correlation with stable fly oviposition, larval development, and adult fitness en_US
dc.type Article (publisher version) en_US
dc.date.published 2014 en_US
dc.citation.doi 10.3389/fmicb.2014.00590 en_US
dc.citation.epage 9 en_US
dc.citation.issue Article 590 en_US
dc.citation.jtitle Frontiers in Microbiology en_US
dc.citation.spage 1 en_US
dc.citation.volume 5 en_US
dc.contributor.authoreid lzurek en_US

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