Select cardiac copper chaperone proteins are up-regulated by dietary copper deficiency

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Show simple item record Getz, Jean 2009-05-19T13:46:16Z 2009-05-19T13:46:16Z 2009-05-19T13:46:16Z
dc.description.abstract Copper deficiency has been linked with many health problems, among them cardiac hypertrophy. Because of its potential for causing oxidative damage, copper within the cell must be bound to chaperone proteins. In this thesis, we examined the role of dietary copper deficiency in the regulation of select copper chaperone proteins in cardiac tissue of rats. Sixteen weanling male Long-Evans rats were randomized into treatment groups, one group receiving a copper deficient diet (< 1 mg Cu/kg diet) and one group receiving a diet containing adequate copper (6 mg Cu/kg diet) for 5 weeks. Rats were sacrificed and a small blood sample was removed to determine hematocrit. Also, heart and liver tissues were removed for subsequent analysis. Rats fed the copper deficient diet had lower body weights but greater heart weights and heart:body weight. Hematocrit levels and liver copper concentrations were markedly decreased in copper deficient rats. These variables indicated that the copper deficient diet did in fact induce a copper deficiency in these animals. Non-myofibrillar proteins from the hearts were removed and separated by SDS-PAGE. Western Blotting was used to determine the concentrations of CTR1, CCS, Cox17, SCO1, Cox1 and Cox4. No changes were observed in the concentrations of CTR1 and Cox17. CCS and SCO1 were up-regulated as a result of copper deficiency, while Cox1 and Cox4 were both down-regulated. However, use of another antibody against Cox subunits suggested that only the nuclear encoded subunits including subunit IV were decreased, but not subunits I and II. These data provide new insight into the cardiac hypertrophy observed in copper deficiency, which suggests that select chaperone proteins may be up-regulated by a dietary copper deficiency. en
dc.language.iso en_US en
dc.publisher Kansas State University en
dc.subject Copper en
dc.subject Copper Deficiency en
dc.subject Heart Disease en
dc.subject Cardiac Hypertrophy en
dc.subject Chaperone Proteins en
dc.title Select cardiac copper chaperone proteins are up-regulated by dietary copper deficiency en
dc.type Thesis en Master of Science en
dc.description.level Masters en
dc.description.department Department of Human Nutrition en
dc.description.advisor Denis M. Medeiros en
dc.subject.umi Biology, General (0306) en
dc.subject.umi Chemistry, Biochemistry (0487) en
dc.subject.umi Health Sciences, General (0566) en
dc.subject.umi Health Sciences, Nutrition (0570) en
dc.subject.umi Health Sciences, Pathology (0571) en 2009 en May en

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