Select cardiac copper chaperone proteins are up-regulated by dietary copper deficiency

dc.contributor.authorGetz, Jean
dc.date.accessioned2009-05-19T13:46:16Z
dc.date.available2009-05-19T13:46:16Z
dc.date.graduationmonthMayen
dc.date.issued2009-05-19T13:46:16Z
dc.date.published2009en
dc.description.abstractCopper deficiency has been linked with many health problems, among them cardiac hypertrophy. Because of its potential for causing oxidative damage, copper within the cell must be bound to chaperone proteins. In this thesis, we examined the role of dietary copper deficiency in the regulation of select copper chaperone proteins in cardiac tissue of rats. Sixteen weanling male Long-Evans rats were randomized into treatment groups, one group receiving a copper deficient diet (< 1 mg Cu/kg diet) and one group receiving a diet containing adequate copper (6 mg Cu/kg diet) for 5 weeks. Rats were sacrificed and a small blood sample was removed to determine hematocrit. Also, heart and liver tissues were removed for subsequent analysis. Rats fed the copper deficient diet had lower body weights but greater heart weights and heart:body weight. Hematocrit levels and liver copper concentrations were markedly decreased in copper deficient rats. These variables indicated that the copper deficient diet did in fact induce a copper deficiency in these animals. Non-myofibrillar proteins from the hearts were removed and separated by SDS-PAGE. Western Blotting was used to determine the concentrations of CTR1, CCS, Cox17, SCO1, Cox1 and Cox4. No changes were observed in the concentrations of CTR1 and Cox17. CCS and SCO1 were up-regulated as a result of copper deficiency, while Cox1 and Cox4 were both down-regulated. However, use of another antibody against Cox subunits suggested that only the nuclear encoded subunits including subunit IV were decreased, but not subunits I and II. These data provide new insight into the cardiac hypertrophy observed in copper deficiency, which suggests that select chaperone proteins may be up-regulated by a dietary copper deficiency.en
dc.description.advisorDenis M. Medeirosen
dc.description.degreeMaster of Scienceen
dc.description.departmentDepartment of Human Nutritionen
dc.description.levelMastersen
dc.identifier.urihttp://hdl.handle.net/2097/1489
dc.language.isoen_USen
dc.publisherKansas State Universityen
dc.subjectCopperen
dc.subjectCopper Deficiencyen
dc.subjectHeart Diseaseen
dc.subjectCardiac Hypertrophyen
dc.subjectChaperone Proteinsen
dc.subject.umiBiology, General (0306)en
dc.subject.umiChemistry, Biochemistry (0487)en
dc.subject.umiHealth Sciences, General (0566)en
dc.subject.umiHealth Sciences, Nutrition (0570)en
dc.subject.umiHealth Sciences, Pathology (0571)en
dc.titleSelect cardiac copper chaperone proteins are up-regulated by dietary copper deficiencyen
dc.typeThesisen

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