Assessment of mastitic infection in bovine milk using ATP bioluminescence

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dc.contributor.author Meyer, M. J.
dc.contributor.author Sukup, D.
dc.contributor.author Schmidt, Karen A.
dc.contributor.author Shirley, John E.
dc.date.accessioned 2011-05-04T14:44:00Z
dc.date.available 2011-05-04T14:44:00Z
dc.date.issued 2011-05-04
dc.identifier.uri http://hdl.handle.net/2097/8597
dc.description.abstract Few choices exist for a mobile, rapid, and nonsubjective assessment of mastitic infection in bovine milk. This project evaluated the effectiveness of using the Biotrace® raw milk quality ATP bioluminescence assay to serve this role. Milk samples with various somatic cell counts (13,000 - 2,500,000) and signs of mastitic infection were obtained from the Kansas State University Dairy Teaching and Research Center. Within 24 hr, raw milk samples were evaluated for microbial numbers and relative light units (RLU). The printed test procedure was modified to evaluate accurately clinical mastitic milk samples. As somatic cell count increased in raw milk, the RLU value increased. In addition, RLU values differentiated among milk samples with various levels of mastitic infection (none, subclinical, and clinical). Repeatability of the ATP bio-luminescence method was very good (CV = 4.76%). These results suggest that the Biotrace® raw milk quality test kit can served effectively as a nonsubjective, rapid assay to determine the degree of mastitic infection in bovine milk. en_US
dc.publisher Kansas Agricultural Experiment Station and Cooperative Extension Service. en_US
dc.relation.isPartOf Dairy Day, 1998 en_US
dc.relation.isPartOf Kansas Agricultural Experiment Station contribution; no. 99-158-S en_US
dc.relation.isPartOf Report of progress (Kansas Agricultural Experiment Station and Cooperative Extension Service); 821 en_US
dc.subject Mastitis en_US
dc.subject Somatic cell count en_US
dc.subject ATP bioluminescence en_US
dc.title Assessment of mastitic infection in bovine milk using ATP bioluminescence en_US
dc.type Conference paper en_US
dc.date.published 1998 en_US
dc.citation.epage 35 en_US
dc.citation.spage 32 en_US
dc.description.conference Dairy Day, 1998, Kansas State University, Manhattan, KS, 1998
dc.contributor.authoreid kschmidt en_US
dc.contributor.authoreid jshirley en_US


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