Pectinmethylesterase from the rice weevil, Sitophilus oryzae: cDNA isolation and sequencing, genetic origin, and expression of the recombinant enzyme

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dc.contributor.author Shen, Zhicheng
dc.contributor.author Pappan, Kirk
dc.contributor.author Mutti, Navdeep S.
dc.contributor.author He, Qi-Jiong
dc.contributor.author Denton, Michael
dc.contributor.author Zhang, Yu
dc.contributor.author Kanost, Michael R.
dc.contributor.author Reese, John C.
dc.contributor.author Reeck, Gerald R.
dc.date.accessioned 2008-05-30T19:53:07Z
dc.date.available 2008-05-30T19:53:07Z
dc.date.issued 2008-05-30T19:53:07Z
dc.identifier.uri http://hdl.handle.net/2097/834
dc.description.abstract A cDNA clone encoding pectinmethylesterase of the rice weevil, Sitophilus oryzae (L.) has been isolated and sequenced. The cDNA clone was expressed in cultured insect cells and active pectinmethylesterase was purified from the culture medium, thus confirming that the cDNA encodes pectinmethylesterase. In situ hybridization indicated that the enzyme's transcript was present in the midgut. Weevils treated with tetracycline so that they lack genes of known symbiotic organisms still contained the pectinmethylesterase gene, indicating that the gene is encoded by the rice weevil genome. The rice weevil enzyme is most similar in sequence to bacterial pectinmethylesterases. Given this and the enzyme's apparently rather general absence from animal species, we suggest the possibility that this gene was transferred horizontally to an ancient weevil, possibly from a bacterial symbiont, and exists in Sitophilus species now as a result of that ancestral horizontal transfer. en
dc.subject Pectinase en
dc.subject Pectin esterase en
dc.subject Polygalacturonase en
dc.subject Gut en
dc.subject Digestive enzyme en
dc.subject Pectin en
dc.subject Symbiont en
dc.subject Wolbachia en
dc.subject SOPE en
dc.subject Gene transfer en
dc.title Pectinmethylesterase from the rice weevil, Sitophilus oryzae: cDNA isolation and sequencing, genetic origin, and expression of the recombinant enzyme en
dc.type Article (publisher version) en
dc.date.published 2005
dc.citation.epage 9
dc.citation.issue 21
dc.citation.jtitle Journal of insect science
dc.citation.spage 1
dc.citation.volume 5


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