Pectinmethylesterase from the rice weevil, Sitophilus oryzae: cDNA isolation and sequencing, genetic origin, and expression of the recombinant enzyme

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dc.contributor.author Shen, Zhicheng
dc.contributor.author Pappan, Kirk
dc.contributor.author Mutti, Navdeep S.
dc.contributor.author He, Qi-Jiong
dc.contributor.author Denton, Michael
dc.contributor.author Zhang, Yu
dc.contributor.author Kanost, Michael R.
dc.contributor.author Reese, John C.
dc.contributor.author Reeck, Gerald R.
dc.date.accessioned 2008-05-30T19:53:07Z
dc.date.available 2008-05-30T19:53:07Z
dc.date.issued 2008-05-30
dc.identifier.uri http://hdl.handle.net/2097/834
dc.description Citation: Shen Z, Pappan K, Mutti NS, He Q-J, Denton M, Zhang Y, Kanost MR, Reese JC, & Reeck, GR. (2005). Journal of Insect Science, 5(21), 1-9. https://doi.org/10.1673/031.005.2101
dc.description.abstract A cDNA clone encoding pectinmethylesterase of the rice weevil, Sitophilus oryzae (L.) has been isolated and sequenced. The cDNA clone was expressed in cultured insect cells and active pectinmethylesterase was purified from the culture medium, thus confirming that the cDNA encodes pectinmethylesterase. In situ hybridization indicated that the enzyme's transcript was present in the midgut. Weevils treated with tetracycline so that they lack genes of known symbiotic organisms still contained the pectinmethylesterase gene, indicating that the gene is encoded by the rice weevil genome. The rice weevil enzyme is most similar in sequence to bacterial pectinmethylesterases. Given this and the enzyme's apparently rather general absence from animal species, we suggest the possibility that this gene was transferred horizontally to an ancient weevil, possibly from a bacterial symbiont, and exists in Sitophilus species now as a result of that ancestral horizontal transfer. en
dc.relation.uri https://doi.org/10.1673/031.005.2101
dc.rights This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.rights.uri https://rightsstatements.org/page/InC/1.0/?language=en
dc.subject Pectinase en
dc.subject Pectin esterase en
dc.subject Polygalacturonase en
dc.subject Gut en
dc.subject Digestive enzyme en
dc.subject Pectin en
dc.subject Symbiont en
dc.subject Wolbachia en
dc.subject SOPE en
dc.subject Gene transfer en
dc.title Pectinmethylesterase from the rice weevil, Sitophilus oryzae: cDNA isolation and sequencing, genetic origin, and expression of the recombinant enzyme en
dc.type Text en
dc.date.published 2005
dc.citation.doi 10.1673/031.005.2101
dc.citation.epage 9
dc.citation.issn 1536-2442
dc.citation.issue 21
dc.citation.jtitle Journal of insect science
dc.citation.spage 1
dc.citation.volume 5
dc.citation Shen Z, Pappan K, Mutti NS, He Q-J, Denton M, Zhang Y, Kanost MR, Reese JC, & Reeck, GR. (2005). Journal of Insect Science, 5(21), 1-9. https://doi.org/10.1673/031.005.2101
dc.description.version Article (publisher version)


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This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). Except where otherwise noted, the use of this item is bound by the following: This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).

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