Cloning and characterization of feline islet glucokinase

K-REx Repository

Show simple item record

dc.contributor.author Lindbloom-Hawley, Sara
dc.contributor.author LeCluyse, Michelle
dc.contributor.author Vandersande, Vanessa
dc.contributor.author Lushington, Gerald Henry
dc.contributor.author Schermerhorn, Thomas
dc.date.accessioned 2018-11-13T20:52:38Z
dc.date.available 2018-11-13T20:52:38Z
dc.date.issued 2014-06-10
dc.identifier.uri http://hdl.handle.net/2097/39271
dc.description Citation: Lindbloom-Hawley, S., LeCluyse, M., Vandersande, V., Lushington, G. H., & Schermerhorn, T. (2014). Cloning and characterization of feline islet glucokinase. BMC Veterinary Research, 10(1), 130. https://doi.org/10.1186/1746-6148-10-130
dc.description.abstract Glucokinase (GK) is a metabolic enzyme encoded by the GCK gene and expressed in glucose-sensitive tissues, principally pancreatic islets cell and hepatocytes. The GK protein acts in pancreatic islets as a “glucose sensor” that couples fluctuations in the blood glucose concentration to changes in cellular function and insulin secretion. GCK and GK have proposed importance in the development and progression of diabetes mellitus and are potential therapeutic targets for diabetes treatment. The study was undertaken to determine the nucleotide sequence of feline pancreatic GK cDNA, predict the amino acid sequence and structure of the feline GK protein, and perform comparative bioinformatic analysis of feline cDNA and protein. Routine PCR techniques were used with cDNA from feline pancreas. Clones were assembled to obtain the full length cDNA. Protein prediction and modeling were performed using bioinformatic tools.
dc.relation.uri https://doi.org/10.1186/1746-6148-10-130
dc.rights Attribution 2.0 Generic (CC BY 2.0)
dc.rights.uri http://creativecommons.org/licenses/by/2.0
dc.subject Glucose sensor
dc.subject Pancreas
dc.subject Carbohydrate metabolism
dc.subject Hexokinase
dc.subject Gene expression
dc.title Cloning and characterization of feline islet glucokinase
dc.type Text
dc.date.published 2014
dc.citation.doi 10.1186/1746-6148-10-130
dc.citation.issn 1746-6148
dc.citation.issue 1
dc.citation.jtitle BMC Veterinary Research
dc.citation.volume 10
dc.citation Lindbloom-Hawley, S., LeCluyse, M., Vandersande, V., Lushington, G. H., & Schermerhorn, T. (2014). Cloning and characterization of feline islet glucokinase. BMC Veterinary Research, 10(1), 130. https://doi.org/10.1186/1746-6148-10-130
dc.description.version Article:Version of Record (VOR)


Files in this item

This item appears in the following Collection(s)

Show simple item record

Attribution 2.0 Generic (CC BY 2.0) Except where otherwise noted, the use of this item is bound by the following: Attribution 2.0 Generic (CC BY 2.0)

Search K-REx


Advanced Search

Browse

My Account

Statistics








Center for the

Advancement of Digital

Scholarship

118 Hale Library

Manhattan KS 66506


(785) 532-7444

cads@k-state.edu