Cloning and characterization of feline islet glucokinase

dc.citationLindbloom-Hawley, S., LeCluyse, M., Vandersande, V., Lushington, G. H., & Schermerhorn, T. (2014). Cloning and characterization of feline islet glucokinase. BMC Veterinary Research, 10(1), 130. https://doi.org/10.1186/1746-6148-10-130
dc.citation.doi10.1186/1746-6148-10-130
dc.citation.issn1746-6148
dc.citation.issue1
dc.citation.jtitleBMC Veterinary Research
dc.citation.volume10
dc.contributor.authorLindbloom-Hawley, Sara
dc.contributor.authorLeCluyse, Michelle
dc.contributor.authorVandersande, Vanessa
dc.contributor.authorLushington, Gerald Henry
dc.contributor.authorSchermerhorn, Thomas
dc.date.accessioned2018-11-13T20:52:38Z
dc.date.available2018-11-13T20:52:38Z
dc.date.issued2014-06-10
dc.date.published2014
dc.descriptionCitation: Lindbloom-Hawley, S., LeCluyse, M., Vandersande, V., Lushington, G. H., & Schermerhorn, T. (2014). Cloning and characterization of feline islet glucokinase. BMC Veterinary Research, 10(1), 130. https://doi.org/10.1186/1746-6148-10-130
dc.description.abstractGlucokinase (GK) is a metabolic enzyme encoded by the GCK gene and expressed in glucose-sensitive tissues, principally pancreatic islets cell and hepatocytes. The GK protein acts in pancreatic islets as a “glucose sensor” that couples fluctuations in the blood glucose concentration to changes in cellular function and insulin secretion. GCK and GK have proposed importance in the development and progression of diabetes mellitus and are potential therapeutic targets for diabetes treatment. The study was undertaken to determine the nucleotide sequence of feline pancreatic GK cDNA, predict the amino acid sequence and structure of the feline GK protein, and perform comparative bioinformatic analysis of feline cDNA and protein. Routine PCR techniques were used with cDNA from feline pancreas. Clones were assembled to obtain the full length cDNA. Protein prediction and modeling were performed using bioinformatic tools.
dc.description.versionArticle:Version of Record (VOR)
dc.identifier.urihttp://hdl.handle.net/2097/39271
dc.relation.urihttps://doi.org/10.1186/1746-6148-10-130
dc.rightsAttribution 2.0 Generic (CC BY 2.0)
dc.rights.urihttp://creativecommons.org/licenses/by/2.0
dc.subjectGlucose sensor
dc.subjectPancreas
dc.subjectCarbohydrate metabolism
dc.subjectHexokinase
dc.subjectGene expression
dc.titleCloning and characterization of feline islet glucokinase
dc.typeText

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