Membrane-Chromatography-Reactor-System for the Continuous Synthesis of Galactosyl-Oligosaccharides

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dc.contributor.author Engel, Larisa
dc.contributor.author Ebrahimi, Mehrdad
dc.contributor.author Czermak, Peter
dc.date.accessioned 2010-04-05T21:40:17Z
dc.date.available 2010-04-05T21:40:17Z
dc.date.issued 2008-04-15
dc.identifier.uri http://hdl.handle.net/2097/3509
dc.description.abstract Galactosyl-oligosaccharides (GOS) are non-digestible oligosaccharides which are qualified as functional foods. GOS are enzymatically synthesized from lactose by transgalactosylation catalyzed by the enzyme β-galactosidase. In this study, a method for immobilization of β-galactosidase from Kluyveromyces lactis on a chromatography membrane was developed. The immobilization was performed at 4, 15 and 40°C. A strong basic anion exchange membrane was investigated. In static experiments the highest enzyme activity was measured at an immobilization procedure temperature of 15°C. The immobilization on the chromatography membranes was found to be very rapid likely due to ionic adsorption. The synthesis of GOS was carried out in a Continuous Membrane Chromatography Reactor System (CMCRS) at 40°C and pH 7.0 using 20 wt% initial lactose concentration. The chromatography membranes proved to be a good support for the continuous process at high convective flow rates in the enzyme reactor system. Up to 82 % lactose conversion with 24 % GOS yield was achieved at different fluxes. The corresponding reactor productivity for the production of GOS from lactose in the CMCRS was 98.7 g GOS per hour and cubic centimeter membrane volume, which significantly exceeds previously reported results. en_US
dc.relation.uri http://doi.org/10.1016/j.desal.2007.04.078 en_US
dc.rights This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.rights.uri https://rightsstatements.org/page/InC/1.0/
dc.subject Immobilization en_US
dc.subject Galactosyl-oligosaccharides en_US
dc.subject Membrane chromatography en_US
dc.title Membrane-Chromatography-Reactor-System for the Continuous Synthesis of Galactosyl-Oligosaccharides en_US
dc.type Text en_US
dc.date.published 2008 en_US
dc.citation.doi 10.1016/j.desal.2007.04.078 en_US
dc.citation.epage 51 en_US
dc.citation.issue 1-3 en_US
dc.citation.jtitle Desalination en_US
dc.citation.spage 46 en_US
dc.citation.volume 224 en_US
dc.contributor.authoreid pczermak en_US
dc.description.version Article (author version)


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This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). Except where otherwise noted, the use of this item is bound by the following: This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).

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