Membrane-Chromatography-Reactor-System for the Continuous Synthesis of Galactosyl-Oligosaccharides

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dc.contributor.author Engel, Larisa
dc.contributor.author Ebrahimi, Mehrdad
dc.contributor.author Czermak, Peter
dc.date.accessioned 2010-04-05T21:40:17Z
dc.date.available 2010-04-05T21:40:17Z
dc.date.issued 2010-04-05T21:40:17Z
dc.identifier.uri http://hdl.handle.net/2097/3509
dc.description.abstract Galactosyl-oligosaccharides (GOS) are non-digestible oligosaccharides which are qualified as functional foods. GOS are enzymatically synthesized from lactose by transgalactosylation catalyzed by the enzyme β-galactosidase. In this study, a method for immobilization of β-galactosidase from Kluyveromyces lactis on a chromatography membrane was developed. The immobilization was performed at 4, 15 and 40°C. A strong basic anion exchange membrane was investigated. In static experiments the highest enzyme activity was measured at an immobilization procedure temperature of 15°C. The immobilization on the chromatography membranes was found to be very rapid likely due to ionic adsorption. The synthesis of GOS was carried out in a Continuous Membrane Chromatography Reactor System (CMCRS) at 40°C and pH 7.0 using 20 wt% initial lactose concentration. The chromatography membranes proved to be a good support for the continuous process at high convective flow rates in the enzyme reactor system. Up to 82 % lactose conversion with 24 % GOS yield was achieved at different fluxes. The corresponding reactor productivity for the production of GOS from lactose in the CMCRS was 98.7 g GOS per hour and cubic centimeter membrane volume, which significantly exceeds previously reported results. en_US
dc.relation.uri http://www.elsevier.com/wps/find/journaldescription.cws_home/502683/description en_US
dc.subject Immobilization en_US
dc.subject Galactosyl-oligosaccharides en_US
dc.subject Membrane chromatography en_US
dc.title Membrane-Chromatography-Reactor-System for the Continuous Synthesis of Galactosyl-Oligosaccharides en_US
dc.type Article (author version) en_US
dc.date.published 2008 en_US
dc.citation.doi 10.1016/j.desal.2007.04.078 en_US
dc.citation.epage 51 en_US
dc.citation.issue 1-3 en_US
dc.citation.jtitle Desalination en_US
dc.citation.spage 46 en_US
dc.citation.volume 224 en_US
dc.contributor.authoreid pczermak en_US


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