Membrane-Chromatography-Reactor-System for the Continuous Synthesis of Galactosyl-Oligosaccharides

dc.citation.doi10.1016/j.desal.2007.04.078en_US
dc.citation.epage51en_US
dc.citation.issue1-3en_US
dc.citation.jtitleDesalinationen_US
dc.citation.spage46en_US
dc.citation.volume224en_US
dc.contributor.authorEngel, Larisa
dc.contributor.authorEbrahimi, Mehrdad
dc.contributor.authorCzermak, Peter
dc.contributor.authoreidpczermaken_US
dc.date.accessioned2010-04-05T21:40:17Z
dc.date.available2010-04-05T21:40:17Z
dc.date.issued2008-04-15
dc.date.published2008en_US
dc.description.abstractGalactosyl-oligosaccharides (GOS) are non-digestible oligosaccharides which are qualified as functional foods. GOS are enzymatically synthesized from lactose by transgalactosylation catalyzed by the enzyme β-galactosidase. In this study, a method for immobilization of β-galactosidase from Kluyveromyces lactis on a chromatography membrane was developed. The immobilization was performed at 4, 15 and 40°C. A strong basic anion exchange membrane was investigated. In static experiments the highest enzyme activity was measured at an immobilization procedure temperature of 15°C. The immobilization on the chromatography membranes was found to be very rapid likely due to ionic adsorption. The synthesis of GOS was carried out in a Continuous Membrane Chromatography Reactor System (CMCRS) at 40°C and pH 7.0 using 20 wt% initial lactose concentration. The chromatography membranes proved to be a good support for the continuous process at high convective flow rates in the enzyme reactor system. Up to 82 % lactose conversion with 24 % GOS yield was achieved at different fluxes. The corresponding reactor productivity for the production of GOS from lactose in the CMCRS was 98.7 g GOS per hour and cubic centimeter membrane volume, which significantly exceeds previously reported results.en_US
dc.description.versionArticle (author version)
dc.identifier.urihttp://hdl.handle.net/2097/3509
dc.relation.urihttp://doi.org/10.1016/j.desal.2007.04.078en_US
dc.rightsThis Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.rights.urihttps://rightsstatements.org/page/InC/1.0/
dc.subjectImmobilizationen_US
dc.subjectGalactosyl-oligosaccharidesen_US
dc.subjectMembrane chromatographyen_US
dc.titleMembrane-Chromatography-Reactor-System for the Continuous Synthesis of Galactosyl-Oligosaccharidesen_US
dc.typeTexten_US

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