Effect of extended postmortem aging and steak location on myofibrillar protein degradation and Warner-Bratzler shear force of beef M. semitendinosus steaks

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dc.contributor.author Phelps, K. J.
dc.contributor.author Drouillard, James S.
dc.contributor.author Silva, M. B.
dc.contributor.author Miranda, L. D. F.
dc.contributor.author Ebarb, S. M.
dc.contributor.author Van Bibber-Krueger, C. L.
dc.contributor.author O'Quinn, Travis G.
dc.contributor.author Gonzalez, John M.
dc.date.accessioned 2016-09-20T17:40:48Z
dc.date.available 2016-09-20T17:40:48Z
dc.identifier.uri http://hdl.handle.net/2097/34125
dc.description Citation: Phelps, K. J., Drouillard, J. S., Silva, M. B., Miranda, L. D. F., Ebarb, S. M., Van Bibber-Krueger, C. L., . . . Gonzalez, J. M. (2016). Effect of extended postmortem aging and steak location on myofibrillar protein degradation and Warner-Bratzler shear force of beef M. semitendinosus steaks. Journal of Animal Science, 94(1), 412-423. doi:10.2527/jas2015-9862
dc.description.abstract The objective of this study was to evaluate the effect of steak location and postmortem aging on cooked meat tenderness and myofibrillar protein degradation of steaks from M. semitendinosus (ST). Following harvest and a 6 d chill period, the left ST was removed from carcasses of crossbred feedlot steers (n = 60, average hot carcass weight 427 +/- 24 kg). Each ST was fabricated into ten 2.54-cm thick steaks originating from the proximal to distal end of the muscle. Steaks cut adjacent to each other were paired, vacuum packaged, and randomly assigned to 7, 14, 21, 42, or 70 d of aging at 2 +/- 1 degrees C. After aging, within each steak pair, steaks were randomly assigned to Warner-Bratzler shear force or myofibrillar proteolysis analysis (calpain activity and desmin and troponin-T degradation). Muscle fiber type and size were also determined at the 2 ends of the muscle. There was no location x d of aging interaction (P = 0.25) for ST steak WBSF. Steak location affected (quadratic, P < 0.01) WBSF. As steaks were fabricated from the proximal to distal end, WBSF values decreased toward the middle of the muscle and then increased toward the distal end. Activity of all calpains and myofibrillar protein proteolysis were unaffected by steak location (P > 0.13). Type I, IIA, and IIX muscle fibers were larger at the proximal end of the muscle than the distal end (P < 0.01). Increasing d of aging improved WBSF (quadratic, P < 0.01) for the duration of the 70 d postmortem period. As d of aging increased, intact calpain-1 activity decreased (quadratic, P < 0.01) with activity detected through 42 d. Day of aging affected autolyzed calpain-1 (linear, P < 0.01) and calpain-2 activity (quadratic, P < 0.01). Through d 70 of aging, the intensity of intact 55 kDa desmin band decreased (linear, P < 0.01), while there was an increase (linear, P < 0.01) in the degraded 38 kDa band. Similarly, d of aging increased troponin-T proteolysis, indicated by a decrease (quadratic, P < 0.01) in intensity of the intact 40 kDa band and an increase (linear, P < 0.01) in the 30 kDa degraded band. Intramuscular WBSF differences are not due to proteolytic activity or myofibrillar degradation and seem related to muscle fiber size. The improvement of ST steak WBSF through 70 d of aging is partly due to continued degradation of desmin and troponin-T. Calpain proteolytic analysis indicates that autolyzed calpain-1 and calpain-2 may be involved in extended postmortem myofibrillar protein proteolysis.
dc.relation.uri https://doi.org/10.2527/jas.2015-9862
dc.rights Copyright © 2016. American Society of Animal Science.
dc.rights.uri http://www.sherpa.ac.uk/romeo/issn/0021-8812/
dc.subject Extended Aging
dc.subject Location
dc.subject M. Semitendinosus
dc.subject Tenderness
dc.subject Calpain
dc.subject Bovine Skeletal-Muscle
dc.title Effect of extended postmortem aging and steak location on myofibrillar protein degradation and Warner-Bratzler shear force of beef M. semitendinosus steaks
dc.type Article
dc.date.published 2016
dc.citation.doi 10.2527/jas.2015-9862
dc.citation.epage 423
dc.citation.issn 0021-8812
dc.citation.issue 1
dc.citation.jtitle Journal of Animal Science
dc.citation.spage 412
dc.citation.volume 94
dc.description.embargo 2017-01
dc.contributor.authoreid jdrouill
dc.contributor.authoreid travisoquinn
dc.contributor.authoreid johngonz


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