A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome

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dc.contributor.author Chapman, J. A.
dc.contributor.author Mascher, M.
dc.contributor.author Buluç, A.
dc.contributor.author Barry, K.
dc.contributor.author Georganas, E.
dc.contributor.author Session, A.
dc.contributor.author Strnadova, V.
dc.contributor.author Jenkins, J.
dc.contributor.author Sehgal, Sunish K.
dc.contributor.author Oliker, L.
dc.contributor.author Schmutz, J.
dc.contributor.author Yelick, K. A.
dc.contributor.author Scholz, U.
dc.contributor.author Waugh, R.
dc.contributor.author Poland, Jesse A.
dc.date.accessioned 2016-04-06T15:14:27Z
dc.date.available 2016-04-06T15:14:27Z
dc.identifier.uri http://hdl.handle.net/2097/32468
dc.description Citation: Chapman, J. A., Mascher, M., Buluç, A., Barry, K., Georganas, E., Session, A., . . . Rokhsar, D. S. (2015). A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome. Genome Biology, 16(1). doi:10.1186/s13059-015-0582-8
dc.description Polyploid species have long been thought to be recalcitrant to whole-genome assembly. By combining high-throughput sequencing, recent developments in parallel computing, and genetic mapping, we derive, de novo, a sequence assembly representing 9.1 Gbp of the highly repetitive 16 Gbp genome of hexaploid wheat, Triticum aestivum, and assign 7.1 Gb of this assembly to chromosomal locations. The genome representation and accuracy of our assembly is comparable or even exceeds that of a chromosome-by-chromosome shotgun assembly. Our assembly and mapping strategy uses only short read sequencing technology and is applicable to any species where it is possible to construct a mapping population. © 2015 Chapman et al. licensee BioMed Central.
dc.description Additional Authors: Muehlbauer, G. J.;Stein, N.;Rokhsar, D. S.
dc.relation.uri http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4373400/
dc.rights Attribution 4.0 International (CC BY 4.0)
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject Accuracy
dc.subject Article
dc.subject Chromatin Assembly And Disassembly
dc.subject Chromosomal Localization
dc.subject Gene Mapping
dc.subject Hexaploidy
dc.title A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome
dc.type Article
dc.date.published 2015
dc.citation.doi 10.1186/s13059-015-0582-8
dc.citation.issn 1474-7596
dc.citation.issue 1
dc.citation.jtitle Genome Biology
dc.citation.spage 17
dc.citation.volume 16
dc.contributor.authoreid jpoland

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