A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome

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dc.contributor.author Chapman, J. A.
dc.contributor.author Mascher, M.
dc.contributor.author Buluç, A.
dc.contributor.author Barry, K.
dc.contributor.author Georganas, E.
dc.contributor.author Session, A.
dc.contributor.author Strnadova, V.
dc.contributor.author Jenkins, J.
dc.contributor.author Sehgal, Sunish K.
dc.contributor.author Oliker, L.
dc.contributor.author Schmutz, J.
dc.contributor.author Yelick, K. A.
dc.contributor.author Scholz, U.
dc.contributor.author Waugh, R.
dc.contributor.author Poland, Jesse A.
dc.date.accessioned 2016-04-06T15:14:27Z
dc.date.available 2016-04-06T15:14:27Z
dc.date.issued 2015-01-31
dc.identifier.uri http://hdl.handle.net/2097/32468
dc.description Citation: Chapman, J. A., Mascher, M., Buluç, A., Barry, K., Georganas, E., Session, A., . . . Rokhsar, D. S. (2015). A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome. Genome Biology, 16(1). doi:10.1186/s13059-015-0582-8
dc.description Polyploid species have long been thought to be recalcitrant to whole-genome assembly. By combining high-throughput sequencing, recent developments in parallel computing, and genetic mapping, we derive, de novo, a sequence assembly representing 9.1 Gbp of the highly repetitive 16 Gbp genome of hexaploid wheat, Triticum aestivum, and assign 7.1 Gb of this assembly to chromosomal locations. The genome representation and accuracy of our assembly is comparable or even exceeds that of a chromosome-by-chromosome shotgun assembly. Our assembly and mapping strategy uses only short read sequencing technology and is applicable to any species where it is possible to construct a mapping population. © 2015 Chapman et al. licensee BioMed Central.
dc.description Additional Authors: Muehlbauer, G. J.;Stein, N.;Rokhsar, D. S.
dc.relation.uri https://doi.org/10.1186/s13059-015-0582-8
dc.rights © 2015 Chapman et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.rights.uri https://www.biomedcentral.com/about/policies/reprints-and-permissions
dc.subject Accuracy
dc.subject Article
dc.subject Chromatin Assembly And Disassembly
dc.subject Chromosomal Localization
dc.subject Gene Mapping
dc.subject Hexaploidy
dc.title A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome
dc.type Text
dc.date.published 2015
dc.citation.doi 10.1186/s13059-015-0582-8
dc.citation.issn 1474-7596
dc.citation.issn 1474-760X
dc.citation.issue 1
dc.citation.jtitle Genome Biology
dc.citation.spage 17
dc.citation.volume 16
dc.citation Chapman, J. A., Mascher, M., Buluç, A., Barry, K., Georganas, E., Session, A., . . . Rokhsar, D. S. (2015). A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome. Genome Biology, 16(1). https://doi.org/10.1186/s13059-015-0582-8
dc.contributor.authoreid jpoland
dc.description.version Article: Version of Record


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© 2015 Chapman et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative
Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain
Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article,
unless otherwise stated. Except where otherwise noted, the use of this item is bound by the following: © 2015 Chapman et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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