Phlebotomus papatasi SP15: mRNA expression variability and amino acid sequence polymorphisms of field populations

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dc.contributor.author Ramalho-Ortigao, Marcelo
dc.contributor.author Coutinho-Abreu, I. V.
dc.contributor.author Balbino, V. Q.
dc.contributor.author Figueiredo, C. A. S.
dc.contributor.author Mukbel, R.
dc.contributor.author Dayem, H.
dc.contributor.author Hanafi, H. A.
dc.contributor.author El-Hossary, S. S.
dc.contributor.author Fawaz, Eedy
dc.contributor.author Abo-Shehada, M.
dc.contributor.author Hoel, D. F.
dc.contributor.author Stayback, G.
dc.contributor.author Wadsworth, M.
dc.contributor.author Shoue, D. A.
dc.contributor.author Abrudan, J.
dc.date.accessioned 2016-04-04T22:46:01Z
dc.date.available 2016-04-04T22:46:01Z
dc.date.issued 2015-05-29
dc.identifier.uri http://hdl.handle.net/2097/32305
dc.description Citation: Ramalho-Ortigao, M., Coutinho-Abreu, I. V., Balbino, V. Q., Figueiredo, C. A. S., Mukbel, R., Dayem, H., . . . McDowell, M. A. (2015). Phlebotomus papatasi SP15: mRNA expression variability and amino acid sequence polymorphisms of field populations. Parasites & Vectors, 8, 14. doi:10.1186/s13071-015-0914-2
dc.description Background: The Phlebotomus papatasi salivary protein PpSP15 was shown to protect mice against Leishmania major, suggesting that incorporation of salivary molecules in multi-component vaccines may be a viable strategy for anti-Leishmania vaccines. Methods: Here, we investigated PpSP15 predicted amino acid sequence variability and mRNA profile of P. papatasi field populations from the Middle East. In addition, predicted MHC class II T-cell epitopes were obtained and compared to areas of amino acid sequence variability within the secreted protein. Results: The analysis of PpSP15 expression from field populations revealed significant intra-and interpopulation variation.. In spite of the variability detected for P. papatasi populations, common epitopes for MHC class II binding are still present and may potentially be used to boost the response against Le. major infections. Conclusions: Conserved epitopes of PpSP15 could potentially be used in the development of a salivary gland antigen-based vaccine.
dc.description Additional Authors: Lobo, N. F.;Mahon, A. R.;Emrich, S. J.;Kamhawi, S.;Collins, F. H.;McDowell, M. A.
dc.relation.uri https://doi.org/10.1186/s13071-015-0914-2
dc.rights © 2015 Ramalho-Ortigão et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject Sand Fly
dc.subject Saliva
dc.subject Ppsp15
dc.subject Leishmaniasis
dc.subject Vaccine
dc.subject Expression
dc.title Phlebotomus papatasi SP15: mRNA expression variability and amino acid sequence polymorphisms of field populations
dc.type Text
dc.date.published 2015
dc.citation.doi 10.1186/s13071-015-0914-2
dc.citation.issn 1756-3305
dc.citation.issue 1
dc.citation.jtitle Parasites & Vectors
dc.citation.spage 14
dc.citation.volume 8
dc.citation Ramalho-Ortigao, M., Coutinho-Abreu, I. V., Balbino, V. Q., Figueiredo, C. A. S., Mukbel, R., Dayem, H., . . . McDowell, M. A. (2015). Phlebotomus papatasi SP15: mRNA expression variability and amino acid sequence polymorphisms of field populations. Parasites & Vectors, 8, 14. doi:10.1186/s13071-015-0914-2
dc.contributor.authoreid mortigao
dc.description.version Article: Version of Record


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© 2015 Ramalho-Ortigão et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Except where otherwise noted, the use of this item is bound by the following: © 2015 Ramalho-Ortigão et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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