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Phlebotomus papatasi SP15: mRNA expression variability and amino acid sequence polymorphisms of field populations

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dc.citationRamalho-Ortigao, M., Coutinho-Abreu, I. V., Balbino, V. Q., Figueiredo, C. A. S., Mukbel, R., Dayem, H., . . . McDowell, M. A. (2015). Phlebotomus papatasi SP15: mRNA expression variability and amino acid sequence polymorphisms of field populations. Parasites & Vectors, 8, 14. doi:10.1186/s13071-015-0914-2
dc.citation.doi10.1186/s13071-015-0914-2
dc.citation.issn1756-3305
dc.citation.issue1
dc.citation.jtitleParasites & Vectors
dc.citation.spage14
dc.citation.volume8
dc.contributor.authorRamalho-Ortigao, Marcelo
dc.contributor.authorCoutinho-Abreu, I. V.
dc.contributor.authorBalbino, V. Q.
dc.contributor.authorFigueiredo, C. A. S.
dc.contributor.authorMukbel, R.
dc.contributor.authorDayem, H.
dc.contributor.authorHanafi, H. A.
dc.contributor.authorEl-Hossary, S. S.
dc.contributor.authorFawaz, Eedy
dc.contributor.authorAbo-Shehada, M.
dc.contributor.authorHoel, D. F.
dc.contributor.authorStayback, G.
dc.contributor.authorWadsworth, M.
dc.contributor.authorShoue, D. A.
dc.contributor.authorAbrudan, J.
dc.contributor.authoreidmortigao
dc.date.accessioned2016-04-04T22:46:01Z
dc.date.available2016-04-04T22:46:01Z
dc.date.issued2015-05-29
dc.date.published2015
dc.descriptionCitation: Ramalho-Ortigao, M., Coutinho-Abreu, I. V., Balbino, V. Q., Figueiredo, C. A. S., Mukbel, R., Dayem, H., . . . McDowell, M. A. (2015). Phlebotomus papatasi SP15: mRNA expression variability and amino acid sequence polymorphisms of field populations. Parasites & Vectors, 8, 14. doi:10.1186/s13071-015-0914-2
dc.descriptionBackground: The Phlebotomus papatasi salivary protein PpSP15 was shown to protect mice against Leishmania major, suggesting that incorporation of salivary molecules in multi-component vaccines may be a viable strategy for anti-Leishmania vaccines. Methods: Here, we investigated PpSP15 predicted amino acid sequence variability and mRNA profile of P. papatasi field populations from the Middle East. In addition, predicted MHC class II T-cell epitopes were obtained and compared to areas of amino acid sequence variability within the secreted protein. Results: The analysis of PpSP15 expression from field populations revealed significant intra-and interpopulation variation.. In spite of the variability detected for P. papatasi populations, common epitopes for MHC class II binding are still present and may potentially be used to boost the response against Le. major infections. Conclusions: Conserved epitopes of PpSP15 could potentially be used in the development of a salivary gland antigen-based vaccine.
dc.descriptionAdditional Authors: Lobo, N. F.;Mahon, A. R.;Emrich, S. J.;Kamhawi, S.;Collins, F. H.;McDowell, M. A.
dc.description.versionArticle: Version of Record
dc.identifier.urihttp://hdl.handle.net/2097/32305
dc.relation.urihttps://doi.org/10.1186/s13071-015-0914-2
dc.rights© 2015 Ramalho-Ortigão et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectSand Fly
dc.subjectSaliva
dc.subjectPpsp15
dc.subjectLeishmaniasis
dc.subjectVaccine
dc.subjectExpression
dc.titlePhlebotomus papatasi SP15: mRNA expression variability and amino acid sequence polymorphisms of field populations
dc.typeText

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