Transfer of Dicamba Tolerance from Sinapis arvensis to Brassica napus via Embryo Rescue and Recurrent Backcross Breeding

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dc.contributor.author Jugulam, Mithila
dc.contributor.author Ziauddin, A.
dc.contributor.author So, K. K. Y.
dc.contributor.author Chen, S.
dc.contributor.author Hall, J. C.
dc.date.accessioned 2016-03-30T01:38:07Z
dc.date.available 2016-03-30T01:38:07Z
dc.date.issued 2015-11-04
dc.identifier.uri http://hdl.handle.net/2097/32210
dc.description Citation: Jugulam M, Ziauddin A, So KKY, Chen S, Hall JC (2015) Transfer of Dicamba Tolerance from Sinapis arvensis to Brassica napus via Embryo Rescue and Recurrent Backcross Breeding. PLoS ONE 10(11): e0141418. https://doi.org/10.1371/journal.pone.0141418
dc.description.abstract Auxinic herbicides (e.g. dicamba) are extensively used in agriculture to selectively control broadleaf weeds. Although cultivated species of Brassicaceae (e.g. Canola) are susceptible to auxinic herbicides, some biotypes of Sinapis arvensis (wild mustard) were found dicamba resistant in Canada. In this research, dicamba tolerance from wild mustard was introgressed into canola through embryo rescue followed by conventional breeding. Intergeneric hybrids between S. arvensis (2n = 18) and B. napus (2n = 38) were produced through embryo rescue. Embryo formation and hybrid plant regeneration was achieved. Transfer of dicamba tolerance from S. arvensis into the hybrid plants was determined by molecular analysis and at the whole plant level. Dicamba tolerance was introgressed into B. napus by backcrossing for seven generations. Homozygous dicamba-tolerant B. napus lines were identified. The ploidy of the hybrid progeny was assessed by flow cytometry. Finally, introgression of the piece of DNA possibly containing the dicamba tolerance gene into B. napus was confirmed using florescence in situ hybridization (FISH). This research demonstrates for the first time stable introgression of dicamba tolerance from S. arvensis into B. napus via in vitro embryo rescue followed by repeated backcross breeding. Creation of dicamba-tolerant B. napus varieties by this approach may have potential to provide options to growers to choose a desirable herbicide-tolerant technology. Furthermore, adoption of such technology facilitates effective weed control, less tillage, and possibly minimize evolution of herbicide resistant weeds.
dc.relation.uri https://doi.org/10.1371/journal.pone.0141418
dc.rights Attribution 4.0 International (CC BY 4.0) © 2015 Jugulam et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject Auxinic Herbicide Resistance
dc.subject Wild Mustard
dc.subject Weed Management
dc.subject Identification
dc.subject Inheritance
dc.subject Broadleaf
dc.title Transfer of Dicamba Tolerance from Sinapis arvensis to Brassica napus via Embryo Rescue and Recurrent Backcross Breeding
dc.type Text
dc.date.published 2015
dc.citation.doi 10.1371/journal.pone.0141418
dc.citation.issn 1932-6203
dc.citation.issue 11
dc.citation.jtitle PLoS One
dc.citation.spage 13
dc.citation.volume 10
dc.citation Jugulam M, Ziauddin A, So KKY, Chen S, Hall JC (2015) Transfer of Dicamba Tolerance from Sinapis arvensis to Brassica napus via Embryo Rescue and Recurrent Backcross Breeding. PLoS ONE 10(11): e0141418. https://doi.org/10.1371/journal.pone.0141418
dc.contributor.authoreid mithila
dc.description.version Article: Version of Record


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Attribution 4.0 International (CC BY 4.0) © 2015 Jugulam et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, the use of this item is bound by the following: Attribution 4.0 International (CC BY 4.0) © 2015 Jugulam et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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