A novel Tenebrio molitor cadherin is a functional receptor for Bacillus thuringiensis cry3aa toxin

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dc.contributor.author Fabrick, Jeff
dc.contributor.author Oppert, Chris
dc.contributor.author Lorenzen, Marce D.
dc.contributor.author Morris, Kaley
dc.contributor.author Oppert, Brenda
dc.contributor.author Jurat-Fuentes, Juan Luis
dc.date.accessioned 2009-11-20T22:33:39Z
dc.date.available 2009-11-20T22:33:39Z
dc.date.issued 2009-11-20T22:33:39Z
dc.identifier.uri http://hdl.handle.net/2097/2180
dc.description.abstract Cry toxins produced by the bacterium Bacillus thuringiensis (Bt) are effective biological insecticides. Cadherin-like proteins have been reported as functional Cry1A toxin receptors in Lepidoptera. Here we present data that demonstrate a coleopteran cadherin is a functional Cry3Aa toxin receptor. The Cry3Aa receptor cadherin was cloned from Tenebrio molitor larval midgut mRNA, and the predicted protein, TmCad1, has domain structure and a putative toxin binding region similar to those in lepidopteran cadherin Bt receptors. A peptide containing the putative toxin binding region from TmCad1 (rTmCad1p) bound specifically to Cry3Aa and promoted the formation of Cry3Aa toxin oligomers, proposed to be mediators of toxicity in lepidopterans. Injection of TmCad1-specific dsRNA into T. molitor larvae resulted in knockdown of TmCad1 transcript and conferred resistance to Cry3Aa toxicity. These data demonstrate the functional role of TmCad1 as a Cry3Aa receptor in T. molitor and reveal similarities between the mode of action of Cry toxins in Lepidoptera and Coleoptera. en_US
dc.rights This research was originally published in Fabrick, J., Oppert, C., Lorenzen, M. D., Oppert, B. and Jurat-Fuentes, J.L. 2009. A novel Tenebrio molitor cadherin is a functional receptor for Bacillus thuringiensis toxin Cry3Aa. J. Biol. Chem., 284:18401-18410. © The American Society for Biochemistry and Molecular Biology en_US
dc.subject Bacillus thuringiensis en_US
dc.subject Cry3Aa en_US
dc.subject Coleoptera en_US
dc.subject Tenebrio molitor en_US
dc.subject Toxin mode of action en_US
dc.subject Insect cadherin en_US
dc.title A novel Tenebrio molitor cadherin is a functional receptor for Bacillus thuringiensis cry3aa toxin en_US
dc.type Article (author version) en_US
dc.date.published 2009 en_US
dc.citation.epage 18410 en_US
dc.citation.issue 27 en_US
dc.citation.jtitle Journal of Biological Chemistry en_US
dc.citation.spage 18401 en_US
dc.citation.volume 284 en_US
dc.contributor.authoreid bso en_US
dc.contributor.authoreid marce en_US

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