Practical use and development of biomérieux TEMPO® system in microbial food safety

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dc.contributor.author Alsaadi, Yousef Saeed
dc.date.accessioned 2014-11-21T14:51:20Z
dc.date.available 2014-11-21T14:51:20Z
dc.date.issued 2014-11-21
dc.identifier.uri http://hdl.handle.net/2097/18708
dc.description.abstract In the food industry, coliform testing is traditionally done by the time consuming and labor intensive plate count method or tube enumeration methods. The TEMPO® system (bioMérieux, Inc.) was developed to improve laboratory efficiency and to replace traditional methods. It uses a miniaturization of the Most Probable Number (MPN) method with 16 tubes with 3 dilutions in one single disposable card. It utilizes two stations: the TEMPO® Preparation station and the TEMPO® Reading station. In this study, the Oxyase® (Oxyase®, Inc.) enzyme was added to TEMPO® CC (Coliforms Count), TEMPO® AC (aerobic colony count) and TEMPO® EC (E. coli Count) methods. Water samples of 1 ml with 0.1 ml of Oxyase® enzyme were compared to samples without the Oxyase® enzyme using the TEMPO® system. Samples were spiked with different levels of coliforms (10, 102, 103 and 104 CFU/ml), stomached (20 sec), and pipetted into the three different TEMPO® media reagents (4 ml) in duplicate and then automatically transferred into the corresponding TEMPO® cards by the TEMPO® preparation station. Counts were obtained using the TEMPO® reading station after 8, 12, 16, 22 and 24 hours at an incubation temperature of 35°C. Results from 20 replicates were compared statistically. Using TEMPO® tests, high counts in food samples (>6 log 10 CFU/ml) can be read in 6±2 hours of incubation using the time-to-detection calibration curve. The TEMPO® system reduces reading time (reading protocol should be changed). There is no need to wait for 22 hours of incubation only 12 hours is required. Oxyrase® enzyme is not needed for the TEMPO® system. en_US
dc.description.sponsorship bioMérieux, Inc. Oxyase®, Inc. en_US
dc.language.iso en_US en_US
dc.publisher Kansas State University en
dc.subject Oxyase® enzyme en_US
dc.subject TEMPO® en_US
dc.subject Indicator organisms en_US
dc.subject BbioMérieux en_US
dc.subject Aerobic colony count en_US
dc.subject E. coli Count en_US
dc.subject Coliforms count en_US
dc.title Practical use and development of biomérieux TEMPO® system in microbial food safety en_US
dc.type Dissertation en_US
dc.description.degree Doctor of Philosophy en_US
dc.description.level Doctoral en_US
dc.description.department Department of Food Science en_US
dc.description.advisor Daniel Y.C. Fung en_US
dc.subject.umi Food Science (0359) en_US
dc.subject.umi Microbiology (0410) en_US
dc.date.published 2014 en_US
dc.date.graduationmonth December en_US


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