Dose dependent effects of nitrate supplementation on cardiovascular control and microvascular oxygenation dynamics in healthy rats

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Show simple item record Ferguson, Scott K. Hirai, Daniel M. Copp, Steven W. Holdsworth, Clark T. Allen, Jason D. Jones, Andrew M. Musch, Timothy I. Poole, David C. 2014-07-29T19:34:14Z 2014-07-29T19:34:14Z 2014-07-29
dc.description.abstract High dose nitrate (NO3−) supplementation via beetroot juice (BR, 1 mmol/kg/day) lowers mean arterial blood pressure (MAP) and improves skeletal muscle blood flow and O2 delivery/utilization matching thereby raising microvascular O2 pressure (PO2mv). We tested the hypothesis that a low dose of NO3− supplementation, consistent with a diet containing NO3− rich vegetables (BRLD, 0.3 mmol/kg/day), would be sufficient to cause these effects. Male Sprague–Dawley rats were administered a low dose of NO3− (0.3 mmol/kg/day; n = 12), a high dose (1 mmol/kg/day; BRHD, n = 6) or tap water (control, n = 10) for 5 days. MAP, heart rate (HR), blood flow (radiolabeled microspheres) and vascular conductance (VC) were measured during submaximal treadmill exercise (20 m/min, 5% grade, equivalent to ∼60% of maximal O2 uptake). Subsequently, PO2mv (phosphorescence quenching) was measured at rest and during 180 s of electrically-induced twitch contractions (1 Hz, ∼6 V) of the surgically-exposed spinotrapezius muscle. BRLD and BRHD lowered resting (control: 139 ± 4, BRLD: 124 ± 5, BRHD: 128 ± 9 mmHg, P < 0.05, BRLD vs. control) and exercising (control: 138 ± 3, BRLD: 126 ± 4, BRHD: 125 ± 5 mmHg, P < 0.05) MAP to a similar extent. For BRLD this effect occurred in the absence of altered exercising hindlimb muscle(s) blood flow or spinotrapezius PO2mv (rest and across the transient response at the onset of contractions, all P > 0.05), each of which increased significantly for the BRHD condition (all P < 0.05). Whereas BRHD slowed the PO2mv kinetics significantly (i.e., >mean response time, MRT; control: 16.6 ± 2.1, BRHD: 23.3 ± 4.7 s) following the onset of contractions compared to control, in the BRLD group this effect did not reach statistical significance (BRLD: 20.9 ± 1.9 s, P = 0.14). These data demonstrate that while low dose NO3− supplementation lowers MAP during exercise it does so in the absence of augmented muscle blood flow, VC and PO2mv; all of which are elevated at a higher dose. Thus, in healthy animals, a high dose of NO3− supplementation seems necessary to elicit significant changes in exercising skeletal muscle O2 delivery/utilization. en_US
dc.language.iso en_US en_US
dc.relation.uri en_US
dc.subject Nitric oxide en_US
dc.subject Exercise en_US
dc.subject Dietary nitrate en_US
dc.subject Nitrite en_US
dc.subject Mean arterial pressure en_US
dc.subject Blood flow en_US
dc.title Dose dependent effects of nitrate supplementation on cardiovascular control and microvascular oxygenation dynamics in healthy rats en_US
dc.type Article (author version) en_US 2014 en_US
dc.citation.doi doi:10.1016/j.niox.2014.04.007 en_US
dc.citation.epage 58 en_US
dc.citation.jtitle Nitric Oxide en_US
dc.citation.spage 51 en_US
dc.citation.volume 39 en_US
dc.contributor.authoreid swc9999 en_US
dc.contributor.authoreid musch en_US
dc.contributor.authoreid dcpoole en_US

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