Luminol-based bioluminescence imaging of mouse mammary tumors

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dc.contributor.author Alshetaiwi, Hamad S.
dc.contributor.author Balivada, Sivasai
dc.contributor.author Shrestha, Tej Bahadur
dc.contributor.author Pyle, Marla M.
dc.contributor.author Basel, Matthew T.
dc.contributor.author Bossmann, Stefan H.
dc.contributor.author Troyer, Deryl L.
dc.date.accessioned 2014-01-24T17:43:16Z
dc.date.available 2014-01-24T17:43:16Z
dc.date.issued 2014-01-24
dc.identifier.uri http://hdl.handle.net/2097/17057
dc.description.abstract Polymorphonuclear neutrophils (PMNs) are the most abundant circulating blood leukocytes. They are part of the innate immune system and provide a first line of defense by migrating toward areas of inflammation in response to chemical signals released from the site. Some solid tumors, such as breast cancer, also cause recruitment and activation of PMNs and release of myeloperoxidase. In this study, we demonstrate that administration of luminol to mice that have been transplanted with 4T1 mammary tumor cells permits the detection of myeloperoxidase activity, and consequently, the location of the tumor. Luminol allowed detection of activated PMNs only two days after cancer cell transplantation, even though tumors were not yet palpable. In conclusion, luminol-bioluminescence imaging (BLI) can provide a pathway towards detection of solid tumors at an early stage in preclinical tumor models. en_US
dc.language.iso en_US en_US
dc.relation.uri http://www.sciencedirect.com/science/article/pii/S101113441300198X en_US
dc.subject Bioluminescence en_US
dc.subject Chemiluminescence en_US
dc.subject Neutrophil en_US
dc.subject Luminol en_US
dc.subject Cancer detection en_US
dc.subject Murine breast cancer model en_US
dc.title Luminol-based bioluminescence imaging of mouse mammary tumors en_US
dc.type Article (author version) en_US
dc.date.published 2013 en_US
dc.citation.doi doi:10.1016/j.jphotobiol.2013.08.017 en_US
dc.citation.epage 228 en_US
dc.citation.jtitle Journal of Photochemistry and Photobiology B: Biology en_US
dc.citation.spage 223 en_US
dc.citation.volume 127 en_US
dc.contributor.authoreid tbs3 en_US
dc.contributor.authoreid mpyle en_US
dc.contributor.authoreid mbasel en_US
dc.contributor.authoreid sbossman en_US
dc.contributor.authoreid troyer en_US


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