Luminol-based bioluminescence imaging of mouse mammary tumors

dc.citation.doi10.1016/j.jphotobiol.2013.08.017en_US
dc.citation.epage228en_US
dc.citation.jtitleJournal of Photochemistry and Photobiology B: Biologyen_US
dc.citation.spage223en_US
dc.citation.volume127en_US
dc.contributor.authorAlshetaiwi, Hamad S.
dc.contributor.authorBalivada, Sivasai
dc.contributor.authorShrestha, Tej Bahadur
dc.contributor.authorPyle, Marla M.
dc.contributor.authorBasel, Matthew T.
dc.contributor.authorBossmann, Stefan H.
dc.contributor.authorTroyer, Deryl L.
dc.contributor.authoreidtbs3en_US
dc.contributor.authoreidmpyleen_US
dc.contributor.authoreidmbaselen_US
dc.contributor.authoreidsbossmanen_US
dc.contributor.authoreidtroyeren_US
dc.date.accessioned2014-01-24T17:43:16Z
dc.date.available2014-01-24T17:43:16Z
dc.date.issued2013-10-05
dc.date.published2013en_US
dc.description.abstractPolymorphonuclear neutrophils (PMNs) are the most abundant circulating blood leukocytes. They are part of the innate immune system and provide a first line of defense by migrating toward areas of inflammation in response to chemical signals released from the site. Some solid tumors, such as breast cancer, also cause recruitment and activation of PMNs and release of myeloperoxidase. In this study, we demonstrate that administration of luminol to mice that have been transplanted with 4T1 mammary tumor cells permits the detection of myeloperoxidase activity, and consequently, the location of the tumor. Luminol allowed detection of activated PMNs only two days after cancer cell transplantation, even though tumors were not yet palpable. In conclusion, luminol-bioluminescence imaging (BLI) can provide a pathway towards detection of solid tumors at an early stage in preclinical tumor models.en_US
dc.identifier.urihttp://hdl.handle.net/2097/17057
dc.language.isoen_USen_US
dc.relation.urihttp://doi.org/10.1016/j.jphotobiol.2013.08.017en_US
dc.subjectBioluminescenceen_US
dc.subjectChemiluminescenceen_US
dc.subjectNeutrophilen_US
dc.subjectLuminolen_US
dc.subjectCancer detectionen_US
dc.subjectMurine breast cancer modelen_US
dc.titleLuminol-based bioluminescence imaging of mouse mammary tumorsen_US
dc.typeArticle (author version)en_US

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