Quantitative proteomic analysis of the Anopheles gambiae (Diptera: Culicidae) midgut infected with o’nyong–nyong virus

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dc.contributor.author Rider, Mark A.
dc.contributor.author Zou, Jin
dc.contributor.author Vanlandingham, Dana L.
dc.contributor.author Nuckols, John T.
dc.contributor.author Higgs, Stephen
dc.contributor.author Zhang, Qiang
dc.contributor.author Lacey, Michelle
dc.contributor.author Kim, Joohyun
dc.contributor.author Wang, Guangdi
dc.contributor.author Hong, Young S.
dc.date.accessioned 2013-11-15T22:23:43Z
dc.date.available 2013-11-15T22:23:43Z
dc.date.issued 2013-11-15
dc.identifier.uri http://hdl.handle.net/2097/16811
dc.description.abstract Alphaviruses are arthropod-borne pathogens that infect a range of hosts. In humans and other mammals, alphavirus infection can cause severe disease. In mosquito hosts, however, there are generally few symptoms. Little is known about the cellular responses of mosquitoes that allow them to cope with infection. In this investigation, a six-plex tandem mass tagging proteomic approach was used to study protein accumulation changes in the midgut of Anopheles gambiae (Giles) (Diptera: Culicidae) mosquitoes infected with o'nyong-nyong virus (Togaviridae, Alphavirus). Five hundred thirty-six nonredundant proteins were identified. Twenty-two were found in significantly different quantities in infected midguts compared with controls. Of interest, analysis revealed molecular pathways possibly targeted by virus proteins, such as those involving TAF4 and DNA polymerase phi proteins. Also identified was an FK506-binding protein. FK506-binding protein orthologs have been described as conserved host resistance factors, which suppress dengue and West Nile virus infection in human HeLa cells. This investigation constitutes the first study of the midgut-specific proteome of An. gambiae in relation to alphavirus infection. Our findings offer insight into mosquito immunity, including factors that possibly contribute to the different pathological outcomes observed in vertebrate and insect hosts. en_US
dc.language.iso en_US en_US
dc.relation.uri http://www.bioone.org/doi/full/10.1603/ME12155 en_US
dc.rights Permission to archive granted by the Entomological Society of America, October 30, 2013. This article is the copyright property of the Entomological Society of America and may not be used for any commercial or other private purpose without specific written permission of the Entomological Society of America. en_US
dc.subject O'nyong-nyong virus en_US
dc.subject Anopheles gambiae en_US
dc.subject Tandem mass tag en_US
dc.subject FK506-binding protein en_US
dc.subject Midgut en_US
dc.title Quantitative proteomic analysis of the Anopheles gambiae (Diptera: Culicidae) midgut infected with o’nyong–nyong virus en_US
dc.type Article (publisher version) en_US
dc.date.published 2013 en_US
dc.citation.doi doi:10.1603/ME12155 en_US
dc.citation.epage 1088 en_US
dc.citation.issue 5 en_US
dc.citation.jtitle Journal of Medical Entomology en_US
dc.citation.spage 1077 en_US
dc.citation.volume 50 en_US
dc.contributor.authoreid dlvanlan en_US
dc.contributor.authoreid shiggs en_US

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