Quantitative proteomic analysis of the Anopheles gambiae (Diptera: Culicidae) midgut infected with o’nyong–nyong virus

dc.citation.doi10.1603/ME12155en_US
dc.citation.epage1088en_US
dc.citation.issue5en_US
dc.citation.jtitleJournal of Medical Entomologyen_US
dc.citation.spage1077en_US
dc.citation.volume50en_US
dc.contributor.authorRider, Mark A.
dc.contributor.authorZou, Jin
dc.contributor.authorVanlandingham, Dana L.
dc.contributor.authorNuckols, John T.
dc.contributor.authorHiggs, Stephen
dc.contributor.authorZhang, Qiang
dc.contributor.authorLacey, Michelle
dc.contributor.authorKim, Joohyun
dc.contributor.authorWang, Guangdi
dc.contributor.authorHong, Young S.
dc.contributor.authoreiddlvanlanen_US
dc.contributor.authoreidshiggsen_US
dc.date.accessioned2013-11-15T22:23:43Z
dc.date.available2013-11-15T22:23:43Z
dc.date.issued2013-09-01
dc.date.published2013en_US
dc.description.abstractAlphaviruses are arthropod-borne pathogens that infect a range of hosts. In humans and other mammals, alphavirus infection can cause severe disease. In mosquito hosts, however, there are generally few symptoms. Little is known about the cellular responses of mosquitoes that allow them to cope with infection. In this investigation, a six-plex tandem mass tagging proteomic approach was used to study protein accumulation changes in the midgut of Anopheles gambiae (Giles) (Diptera: Culicidae) mosquitoes infected with o'nyong-nyong virus (Togaviridae, Alphavirus). Five hundred thirty-six nonredundant proteins were identified. Twenty-two were found in significantly different quantities in infected midguts compared with controls. Of interest, analysis revealed molecular pathways possibly targeted by virus proteins, such as those involving TAF4 and DNA polymerase phi proteins. Also identified was an FK506-binding protein. FK506-binding protein orthologs have been described as conserved host resistance factors, which suppress dengue and West Nile virus infection in human HeLa cells. This investigation constitutes the first study of the midgut-specific proteome of An. gambiae in relation to alphavirus infection. Our findings offer insight into mosquito immunity, including factors that possibly contribute to the different pathological outcomes observed in vertebrate and insect hosts.en_US
dc.identifier.urihttp://hdl.handle.net/2097/16811
dc.language.isoen_USen_US
dc.relation.urihttp://www.doi.org/10.1603/ME12155en_US
dc.rightsPermission to archive granted by the Entomological Society of America, October 30, 2013. This article is the copyright property of the Entomological Society of America and may not be used for any commercial or other private purpose without specific written permission of the Entomological Society of America.en_US
dc.subjectO'nyong-nyong virusen_US
dc.subjectAnopheles gambiaeen_US
dc.subjectTandem mass tagen_US
dc.subjectFK506-binding proteinen_US
dc.subjectMidguten_US
dc.titleQuantitative proteomic analysis of the Anopheles gambiae (Diptera: Culicidae) midgut infected with o’nyong–nyong virusen_US
dc.typeArticle (publisher version)en_US

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