Porcine vas deferens luminal pH is acutely increased by systemic xylazine administration

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dc.contributor.author Pierucci-Alves, Fernando
dc.contributor.author Duncan, Cameron L.
dc.contributor.author Lillich, James D.
dc.contributor.author Schultz, Bruce D.
dc.date.accessioned 2013-06-27T19:30:43Z
dc.date.available 2013-06-27T19:30:43Z
dc.date.issued 2013-06-27
dc.identifier.uri http://hdl.handle.net/2097/15939
dc.description.abstract Data are accumulating to demonstrate that pH regulation in the male reproductive tract has a vital role in modulating sperm cell fertilizing capacity and, therefore, male fertility. Bicarbonate uptake by sperm cells is required for the achievement of motility levels required for fertilization. Vas deferens epithelial cells can carry out measurable bicarbonate secretion, but the available literature to date reports that the vas deferens luminal content is typically acidic. This study aimed to determine pH in the boar vas deferens lumen and whether modulatory mechanisms exist for regulation of pH in this compartment of the male reproductive tract. A fiber-optic pH probe was used to assess pH in the vas deferens of anesthetized adult boars. The mean pH, derived from multiple measurements at variable positions along the vas deferens lumen, was 7.39 ± 0.09. Furthermore, administration of xylazine, an alpha-2 adrenergic receptor agonist, rapidly (< 10 min) alkalinized the vas deferens lumen in most cases. Since the duct was transected proximal to the site of measurements, the observations rule out the possibility that alkalinization resulted from secretion in more proximal portions of the duct. These results indicate that the boar vas deferens lumen can be alkaline, and suggest that porcine vas deferens epithelia increase net bicarbonate secretion in vivo, following systemic alpha-2 adrenergic stimulation. This secretory response greatly changes the luminal environment to which sperm cells are exposed, which will initiate or enhance motility, and is expected to modulate male fertility. en_US
dc.language.iso en_US en_US
dc.relation.uri http://www.biolreprod.org/content/82/1/132.full en_US
dc.rights Permission to archive granted by the Society for the Study of Reproduction, September 20, 2012. en_US
dc.subject Male reproductive tract en_US
dc.subject Vas deferens en_US
dc.title Porcine vas deferens luminal pH is acutely increased by systemic xylazine administration en_US
dc.type Article (author version) en_US
dc.date.published 2010 en_US
dc.citation.doi doi:10.1095/biolreprod.109.078857 en_US
dc.citation.epage 135 en_US
dc.citation.issue 1 en_US
dc.citation.jtitle Biology of Reproduction en_US
dc.citation.spage 132 en_US
dc.citation.volume 82 en_US
dc.contributor.authoreid fpalves en_US
dc.contributor.authoreid lillich en_US
dc.contributor.authoreid bschultz en_US

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