Porcine vas deferens luminal pH is acutely increased by systemic xylazine administration

dc.citation.doi10.1095/biolreprod.109.078857en_US
dc.citation.epage135en_US
dc.citation.issue1en_US
dc.citation.jtitleBiology of Reproductionen_US
dc.citation.spage132en_US
dc.citation.volume82en_US
dc.contributor.authorPierucci-Alves, Fernando
dc.contributor.authorDuncan, Cameron L.
dc.contributor.authorLillich, James D.
dc.contributor.authorSchultz, Bruce D.
dc.contributor.authoreidfpalvesen_US
dc.contributor.authoreidlillichen_US
dc.contributor.authoreidbschultzen_US
dc.date.accessioned2013-06-27T19:30:43Z
dc.date.available2013-06-27T19:30:43Z
dc.date.issued2013-06-27
dc.date.issued2010-01-01
dc.date.published2010en_US
dc.description.abstractData are accumulating to demonstrate that pH regulation in the male reproductive tract has a vital role in modulating sperm cell fertilizing capacity and, therefore, male fertility. Bicarbonate uptake by sperm cells is required for the achievement of motility levels required for fertilization. Vas deferens epithelial cells can carry out measurable bicarbonate secretion, but the available literature to date reports that the vas deferens luminal content is typically acidic. This study aimed to determine pH in the boar vas deferens lumen and whether modulatory mechanisms exist for regulation of pH in this compartment of the male reproductive tract. A fiber-optic pH probe was used to assess pH in the vas deferens of anesthetized adult boars. The mean pH, derived from multiple measurements at variable positions along the vas deferens lumen, was 7.39 ± 0.09. Furthermore, administration of xylazine, an alpha-2 adrenergic receptor agonist, rapidly (< 10 min) alkalinized the vas deferens lumen in most cases. Since the duct was transected proximal to the site of measurements, the observations rule out the possibility that alkalinization resulted from secretion in more proximal portions of the duct. These results indicate that the boar vas deferens lumen can be alkaline, and suggest that porcine vas deferens epithelia increase net bicarbonate secretion in vivo, following systemic alpha-2 adrenergic stimulation. This secretory response greatly changes the luminal environment to which sperm cells are exposed, which will initiate or enhance motility, and is expected to modulate male fertility.en_US
dc.identifier.urihttp://hdl.handle.net/2097/15939
dc.language.isoen_USen_US
dc.relation.urihttp://www.biolreprod.org/content/82/1/132.fullen_US
dc.rightsPermission to archive granted by the Society for the Study of Reproduction, September 20, 2012.en_US
dc.subjectMale reproductive tracten_US
dc.subjectVas deferensen_US
dc.titlePorcine vas deferens luminal pH is acutely increased by systemic xylazine administrationen_US
dc.typeArticle (author version)en_US

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