Single-copy gene fluorescence in situ hybridization and genome analysis: Acc-2 loci mark evolutionary chromosomal rearrangements in wheat

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dc.contributor.author Danilova, Tatiana V.
dc.contributor.author Friebe, Bernd R.
dc.contributor.author Gill, Bikram S.
dc.date.accessioned 2013-02-27T19:11:00Z
dc.date.available 2013-02-27T19:11:00Z
dc.date.issued 2012-10-02
dc.identifier.uri http://hdl.handle.net/2097/15329
dc.description Citation: Danilova, T. V., Friebe, B., & Gill, B. S. (2012). Single-copy gene fluorescence in situ hybridization and genome analysis: Acc-2 loci mark evolutionary chromosomal rearrangements in wheat. Retrieved from http://krex.ksu.edu
dc.description.abstract Fluorescent in situ hybridization (FISH) is a useful tool for physical mapping of chromosomes and studying evolutionary chromosome rearrangements. Here we report a robust method for single-copy gene FISH for wheat. FISH probes were developed from cDNA of cytosolic acetyl-CoA carboxylase gene (Acc-2) and mapped on chromosomes of bread wheat, Triticum aestivum L. (2n=6x=42, AABBDD), and related diploid and tetraploid species. Another nine full-length cDNA FISH probes were mapped and used to identify chromosomes of wheat species. The Acc-2 probe was detected on the long arms of each of the homoeologous group-3 chromosomes (3A, 3B, and 3D), on 5DL and 4AL of bread wheat, and on homoeologous and nonhomoeologous chromosomes of other species. In the species tested, FISH detected more Acc-2 gene or pseudogene sites than previously found by PCR and Southern hybridization analysis and showed presence/absence polymorphism of Acc-2 sequences. FISH with the Acc-2 probe revealed the 4A-5A translocation, shared by several related diploid and polyploid species and inherited from an ancestral A-genome species, and the T. timopheevii specific 4A[superscript t]-3A[superscript t] translocation. en_US
dc.language.iso en_US en_US
dc.relation.uri https://doi.org/10.1007/s00412-012-0384-7 en_US
dc.rights © Springer-Verlag Berlin Heidelberg 2012. The final publication is available at www.springerlink.com en_US
dc.rights.uri https://www.springer.com/gp/open-access/publication-policies/self-archiving-policy
dc.subject Wheat en_US
dc.subject Single-copy gene fluorescence in situ hybridization en_US
dc.subject Acetyl-CoA carboxylase en_US
dc.subject Karyotype evolution en_US
dc.title Single-copy gene fluorescence in situ hybridization and genome analysis: Acc-2 loci mark evolutionary chromosomal rearrangements in wheat en_US
dc.type Text en_US
dc.date.published 2012 en_US
dc.citation.doi 10.1007/s00412-012-0384-7 en_US
dc.citation.epage 611 en_US
dc.citation.issn 0009-5915
dc.citation.issue 6 en_US
dc.citation.jtitle Chromosoma en_US
dc.citation.spage 597 en_US
dc.citation.volume 121 en_US
dc.citation Danilova, T. V., Friebe, B., & Gill, B. S. (2012). Single-copy gene fluorescence in situ hybridization and genome analysis: Acc-2 loci mark evolutionary chromosomal rearrangements in wheat. Retrieved from http://krex.ksu.edu
dc.contributor.authoreid bsgill en_US
dc.contributor.authoreid friebe en_US
dc.description.version Article: Accepted Manuscript


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