Single-copy gene fluorescence in situ hybridization and genome analysis: Acc-2 loci mark evolutionary chromosomal rearrangements in wheat
dc.citation | Danilova, T. V., Friebe, B., & Gill, B. S. (2012). Single-copy gene fluorescence in situ hybridization and genome analysis: Acc-2 loci mark evolutionary chromosomal rearrangements in wheat. Retrieved from http://krex.ksu.edu | |
dc.citation.doi | 10.1007/s00412-012-0384-7 | en_US |
dc.citation.epage | 611 | en_US |
dc.citation.issn | 0009-5915 | |
dc.citation.issue | 6 | en_US |
dc.citation.jtitle | Chromosoma | en_US |
dc.citation.spage | 597 | en_US |
dc.citation.volume | 121 | en_US |
dc.contributor.author | Danilova, Tatiana V. | |
dc.contributor.author | Friebe, Bernd R. | |
dc.contributor.author | Gill, Bikram S. | |
dc.contributor.authoreid | bsgill | en_US |
dc.contributor.authoreid | friebe | en_US |
dc.date.accessioned | 2013-02-27T19:11:00Z | |
dc.date.available | 2013-02-27T19:11:00Z | |
dc.date.issued | 2012-10-02 | |
dc.date.published | 2012 | en_US |
dc.description | Citation: Danilova, T. V., Friebe, B., & Gill, B. S. (2012). Single-copy gene fluorescence in situ hybridization and genome analysis: Acc-2 loci mark evolutionary chromosomal rearrangements in wheat. Retrieved from http://krex.ksu.edu | |
dc.description.abstract | Fluorescent in situ hybridization (FISH) is a useful tool for physical mapping of chromosomes and studying evolutionary chromosome rearrangements. Here we report a robust method for single-copy gene FISH for wheat. FISH probes were developed from cDNA of cytosolic acetyl-CoA carboxylase gene (Acc-2) and mapped on chromosomes of bread wheat, Triticum aestivum L. (2n=6x=42, AABBDD), and related diploid and tetraploid species. Another nine full-length cDNA FISH probes were mapped and used to identify chromosomes of wheat species. The Acc-2 probe was detected on the long arms of each of the homoeologous group-3 chromosomes (3A, 3B, and 3D), on 5DL and 4AL of bread wheat, and on homoeologous and nonhomoeologous chromosomes of other species. In the species tested, FISH detected more Acc-2 gene or pseudogene sites than previously found by PCR and Southern hybridization analysis and showed presence/absence polymorphism of Acc-2 sequences. FISH with the Acc-2 probe revealed the 4A-5A translocation, shared by several related diploid and polyploid species and inherited from an ancestral A-genome species, and the T. timopheevii specific 4A[superscript t]-3A[superscript t] translocation. | en_US |
dc.description.version | Article: Accepted Manuscript | |
dc.identifier.uri | http://hdl.handle.net/2097/15329 | |
dc.language.iso | en_US | en_US |
dc.relation.uri | https://doi.org/10.1007/s00412-012-0384-7 | en_US |
dc.rights | © Springer-Verlag Berlin Heidelberg 2012. The final publication is available at www.springerlink.com | en_US |
dc.rights.uri | https://www.springer.com/gp/open-access/publication-policies/self-archiving-policy | |
dc.subject | Wheat | en_US |
dc.subject | Single-copy gene fluorescence in situ hybridization | en_US |
dc.subject | Acetyl-CoA carboxylase | en_US |
dc.subject | Karyotype evolution | en_US |
dc.title | Single-copy gene fluorescence in situ hybridization and genome analysis: Acc-2 loci mark evolutionary chromosomal rearrangements in wheat | en_US |
dc.type | Text | en_US |