Different gene expression of skin tissues between mice with weight controlled by either calorie restriction or physical exercise

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dc.contributor.author Lu, Jia
dc.contributor.author Xie, Linglin
dc.contributor.author Sylvester, Jessica
dc.contributor.author Wang, Jiasong
dc.contributor.author Bai, Jianfa
dc.contributor.author Baybutt, Richard
dc.contributor.author Wang, Weiqun
dc.date.accessioned 2008-04-28T20:06:29Z
dc.date.available 2008-04-28T20:06:29Z
dc.date.issued 2008-04-28T20:06:29Z
dc.identifier.uri http://hdl.handle.net/2097/649
dc.description.abstract Cancer prevention by weight control via dietary calorie restriction (DCR) and/or exercise has been demonstrated in animal models. To understand the underlying mechanisms, we compared phorbol ester (TPA)-induced gene expression profiles in DCR- or exercise-treated mouse skin tissues. SENCAR mice were randomly assigned to one of the following groups: ad libitum-fed sedentary control, ad libitum-fed exercise (AE), exercise but pair-fed at the amount of the control (PE), and 20% DCR. After 10 weeks, both body weight and fat composition significantly decreased in the DCR and PE groups compared with the controls. Weight loss was not observed in the AE group due, at least in part, to increased food intake. Among 39,000 transcripts with 45,101 probe sets measured by Affymetrix microarray, we identified 411, 110, and 67 genes that showed > 1.5-fold and significant changes by DCR, AE, and PE, respectively. Gene ontology showed a profound impact on gene expression by DCR in 21 biologic process categories. Although PE and AE had a moderate impact on gene expression, the similarity of gene expression pattern altered by PE was relatively closer to DCR, whereas AE was closer to the control. The results of 22 cancer-related gene expression patterns, especially for certain oncogenes, further supported that PE appeared to be a better alternative than AE to DCR-like cancer prevention. The impact on gene expression pattern was associated with the effect on weight loss (i.e., DCR >> PE > AE). Overall, this study demonstrated for the first time that weight control via decreasing energy intake or increasing energy expenditure resulted in the different modes of gene expression. DCR showed profound inhibitory impact on the expression of genes relevant to cancer risks. Furthermore, exercise along with limited calorie intake appears to be a better method for reducing weight and cancer risk compared with exercise alone. en
dc.rights Permission to reproduce this article has been granted by the Society for Experimental Biology and Medicine en
dc.rights.uri http://www.ebmonline.org en
dc.subject Weight control en
dc.subject Gene expression en
dc.subject Calorie restriction en
dc.subject Exercise en
dc.subject Cancer prevention en
dc.subject Mice en
dc.title Different gene expression of skin tissues between mice with weight controlled by either calorie restriction or physical exercise en
dc.type Article (author version) en
dc.date.published 2007 en
dc.citation.atitle jianfa
dc.citation.epage 480 en
dc.citation.issue 4 en
dc.citation.jtitle Experimental biology and medicine en
dc.citation.spage 473 en
dc.citation.volume 232 en
dc.contributor.authoreid wwang
dc.contributor.authoreid lxi9898
dc.contributor.authoreid baybutt

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