Literak, IvanRadimersky, TomasZurek, LudekCernohlavkova, JitkaGhosh, AnuradhaMaslanova, IvanaSedlacek, IvoSvec, PavelFrolkova, Petra2014-05-092014-05-092013-08-01http://hdl.handle.net/2097/17731Two Gram-positive, catalase-negative bacterial strains were isolated from the cloaca of common kingfishers (Alcedo atthis). Repetitive sequence-based PCR fingerprinting using the (GTG)5 primer grouped these isolates into a single cluster separated from all known enterococcal species. The two strains revealed identical 16S rRNA gene sequences placing them within the genus Enterococcus with Enterococcus aquimarinus LMG 16607T as the closest relative (97.14 % similarity). Further taxonomic investigation using sequencing of the genes for the superoxide dismutase (sodA), phenylalanyl-tRNA synthase alpha subunit (pheS) and the RNA polymerase alpha subunit (rpoA) as well as application of whole-cell protein fingerprinting, automated ribotyping and extensive phenotyping confirmed that both strains belong to the same species. Based on data from this polyphasic study, these strains represent a novel species of the genus Enterococcus, for which the name Enterococcus alcedinis sp. nov. is proposed. The type strain is L34T ( = CCM 8433T = LMG 27164T).en-USThis is an author manuscript that has been accepted for publication in International Journal of Systematic and Evolutionary Microbiology, copyright International Union of Microbiological Societies, but has not been copy-edited, formatted or proofed. Cite this article as appearing in International Journal of Systematic and Evolutionary Microbiology. This version of the manuscript may not be duplicated or reproduced, other than for personal use or within the rule of 'Fair Use of Copyrighted Materials' (section 17, Title 17, US Code), without permission from the copyright owner, International Union of Microbiological Societies. The Society for General Microbiology and the International Union of Microbiological Societies disclaim any responsibility or liability for errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final copy-edited, published article, which is the version of record, can be found at http://ijs.sgmjournals.org, and is freely available without a subscription 24 months after publication.New TaxaFirmicutes and related organismsArticle (author version)