Rong, ShuoLi, DaqiZhang, XueyaoLi, ShengZhu, Kun YanGuo, YapingMa, EnboZhang, Jianzhen2013-04-032013-04-032013-04-03http://hdl.handle.net/2097/15451Citation: Rong, S., Li, D., Zhang, X., Li, S., Zhu, K. Y., Guo, Y., . . . Zhang, J. (2013). RNA interference to reveal roles of β-N-acetylglucosaminidase gene during molting process in Locusta migratoria. Retrieved from http://krex.ksu.eduβ-N-acetylglucosaminidases are crucial enzymes involved in chitin degradation in insects. We identified a β-N-acetylglucosaminidase gene (LmNAG1) from Locusta migratoria. The full-length cDNA of LmNAG1 consists of 2667 nucleotides, including an open reading frame (ORF) of 1845 nucleotides encoding 614 amino acid residues, and 233- and 589-nucleotide non-coding regions at the 5’- and 3’-ends, respectively. Phylogenetic analysis grouped the cDNA-deduced LmNAG1 protein with the enzymatically characterized NAGs in group I. Analyses of stage- and tissue-dependent expression patterns of LmNAG1 were carried out by real-time quantitative PCR. Our results showed that LmNAG1 transcript level in the integument was significantly high in the last two days of the fourth and fifth-instar nymphs. LmNAG1 was highly expressed in foregut and hindgut. RNA interference of LmNAG1 resulted in an effective silence of the gene and a significantly reduced total LmNAG enzyme activity at 48 and 72 h after the injection of LmNAG1 dsRNA. As compared with the control nymphs injected with GFP dsRNA, 50% of the dsLmNAG1 injected nymphs were not able to molt successfully and eventually died. Our results suggest that LmNAG1 plays an essential role in molting process of L. migratoria.en-USThis is the pre-peer-reviewed version of the following article: Rong, S., Li, D.-Q., Zhang, X.-Y., Li, S., Zhu, K. Y., Guo, Y.-P., . . . Zhang, J.-Z. (2013). RNA interference to reveal roles of β-N-acetylglucosaminidase gene during molting process in Locusta migratoria. Insect Science, 20(1), 109–119., which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/j.1744-7917.2012.01573.x/full .https://www.springer.com/gp/open-access/publication-policies/self-archiving-policyLocusta migratoriaChitin degradationβ-N-acetylglucosaminidaseRNAiText