Lenehan, N.A.Usry, J.L.Hastad, C.W.Barker, M.R.Frantz, N.Z.Groesbeck, C.N.James, B.W.Keegan, T.P.Lawrence, K.R.Young, M.G.Tokach, Michael D.Goodband, Robert D.Nelssen, Jim L.DeRouchey, Joel M.Dritz, Steven S.2009-11-092009-11-092009-11-09http://hdl.handle.net/2097/2086A total of 360 pigs (initially 22.2 lb and 31 d of age) was used in a 21-d growth assay. This trial was conducted as a combination of two separate trials in order to simultaneously examine both the true ileal digestible lysine and true ileal digestible threonine requirement and determine the appropriate threonine:lysine ratio. The first part of the trial consisted of five treatments with increasing dietary lysine (1.0. 1.1, 1.2, 1.3 and 1.4% true digestible lysine). The second part consisted of five treatments with increasing dietary threonine (0.66, 0.72, 0.78, 0.84 and 0.91% true ileal digestible threonine). The highest level of both lysine and threonine (1.4% and 0.91% respectively) served as a positive control, and this diet was combined as one treatment to give a total of nine treatments. Average daily gain increased to 1.3% true ileal digestible lysine, and then plateaued, while ADG increased to 0.78% true ileal digestible threonine, suggesting a threonine:lysine ratio of 60% for ADG. Increasing dietary lysine improved F/G linearly through 1.4% true ileal digestible lysine, while F/G improved up to a level of 0.84% true ileal digestible threonine. Using a level of 1.4% true ileal digestible lysine, a threonine:lysine ratio of approximately 60% is implicated for F/G. Amino acid and plasma urea N values were measured on d 10 of the trial. Plasma lysine concentrations were maintained steadily as the true ileal digestible lysine level increased, with a slight increase in plasma lysine concentration observed as the true ileal digestible lysine level increased from 1.3% to 1.4%. A linear increase (P<.0001) in plasma threonine concentration was observed as true ileal digestible threonine increased from 0.66% to 0.91%. Plasma urea N decreased linearly (P<0.0003) with increasing true ileal digestible lysine. As true ileal digestible threonine increased, there was no difference seen in plasma urea N concentration. Following analysis of the data, a true ileal digestible threonine to lysine ratio of 60% is suggested. A second study is in progress where the higher true digestible lysine level of 1.5% is used to verify trial results.ThreonineLysineNursery pigsSwineConference paper