Ekeberg, T.Svenda, M.Seibert, M. M.Abergel, C.Maia, F. R. N. C.Seltzer, V.DePonte, D. P.Aquila, A.Andreasson, J.Iwan, B.Jonsson, O.Westphal, D.Odic, D.Andersson, I.Barty, A.Liang, M.Martin, A. V.Gumprecht, L.Fleckenstein, H.Bajt, S.Barthelmess, M.Coppola, N.Claverie, J. M.Loh, N. D.Bostedt, C.Bozek, J. D.Krzywinski, J.Messerschmidt, M.Bogan, M. J.Hampton, C. Y.Sierra, R. G.Frank, M.Shoeman, R. L.Lomb, L.Foucar, L.Epp, S. W.Rolles, DanielRudenko, ArtemHartmann, R.Hartmann, A.Kimmel, N.Holl, P.Weidenspointner, G.Rudek, B.Erk, B.Kassemeyer, S.Schlichting, I.Struder, L.Ullrich, J.Schmidt, C.Krasniqi, F.Hauser, G.Reich, C.Soltau, H.Schorb, S.Hirsemann, H.Wunderer, C.Graafsma, H.Chapman, H.Hajdu, J.2017-04-102017-04-102016-08-01http://hdl.handle.net/2097/35353Citation: Ekeberg, T., Svenda, M., Seibert, M. M., Abergel, C., Maia, F. R. N. C., Seltzer, V., . . . Hajdu, J. (2016). Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser. Scientific Data, 3. doi:10.1038/sdata.2016.60Free-electron lasers (FEL) hold the potential to revolutionize structural biology by producing X-ray pules short enough to outrun radiation damage, thus allowing imaging of biological samples without the limitation from radiation damage. Thus, a major part of the scientific case for the first FELs was three-dimensional (3D) reconstruction of non-crystalline biological objects. In a recent publication we demonstrated the first 3D reconstruction of a biological object from an X-ray FEL using this technique. The sample was the giant Mimivirus, which is one of the largest known viruses with a diameter of 450 nm. Here we present the dataset used for this successful reconstruction. Data-analysis methods for single-particle imaging at FELs are undergoing heavy development but data collection relies on very limited time available through a highly competitive proposal process. This dataset provides experimental data to the entire community and could boost algorithm development and provide a benchmark dataset for new algorithms.Attribution 4.0 International (CC BY 4.0)https://creativecommons.org/licenses/by/4.0/Article