Detection of Escherichia coli O104 in the feces of feedlot cattle by a multiplex PCR assay designed to target major genetic traits of the virulent hybrid strain responsible for the 2011 German outbreak

Paddock, Zachary DeanBai, JianfaShi, X.Renter, David G.Nagaraja, Tiruvoor G.2013-07-022013-07-022013-05-062013-06-01http://hdl.handle.net/2097/15953A multiplex PCR was designed to detect E. coli O104:H4, a hybrid pathotype of Shiga toxigenic and enteroaggregative E. coli, in cattle feces. A total of 248 fecal samples were tested and 20.6% were positive for the O104 serogroup. The isolates of O104 did not carry genes characteristic of the virulent hybrid strain.en-USEscherichia coliCattleFecesDetection of Escherichia coli O104 in the feces of feedlot cattle by a multiplex PCR assay designed to target major genetic traits of the virulent hybrid strain responsible for the 2011 German outbreakText