Singh, Chingakham R.Lovell, ScottMehzabeen, NurjahanChowdhury, Wasimul Q.Geanes, Eric S.Battaile, Kevin P.Roelofs, Jeroen2014-05-282014-05-282014-05-28http://hdl.handle.net/2097/17799The 26S proteasome is a 2.5 MDa protease dedicated to the degradation of ubiquitinated proteins in eukaryotes. The assembly of this complex containing 66 polypeptides is assisted by at least nine proteasome-specific chaperones. One of these, Nas2, binds to the proteasomal AAA-ATPase subunit Rpt5. The PDZ domain of Nas2 binds to the C-terminal tail of Rpt5; however, it does not require the C-terminus of Rpt5 for binding. Here, the 1.15 Å resolution structure of the PDZ domain of Nas2 is reported. This structure will provide a basis for further insights regarding the structure and function of Nas2 in proteasome assembly.en-USThis open-access article is distributed under the terms of the Creative Commons Attribution Licence http://creativecommons.org/licenses/by/2.0/uk/legalcode, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.26S proteasomeProteasome-assemblyNas2 PDZ domainArticle (publisher version)