Garrett, Karen A.Gomez-Montano, LorenaJumpponen, Ari M.2013-08-072013-08-072013-10-01http://hdl.handle.net/2097/16206Citation: Gomez-Montano, L. Jumpponen, A., Gonzales, M., . . . Garrett, K. (2013). Do bacterial and fungal communities in soils of the Bolivian Altiplano change under shorter fallow periods?. Soil Biology & Biochemistry, 65, 50-59. https://doi.org/10.1016/j.soilbio.2013.04.005This R script performs statistical analyses using sequence data. It goes through all the steps from reading the data into R, merging multiple datasets, calculating diversity metrics, performing statistical analysis such as linear regression and non-metric multidimensional scaling (NMDS), making graphs and saving and exporting them for use in presentations and publications. It illustrates the analyses used in Gomez-Montano et al. 2013, and can be used as a template for other similar analyses. Sections: 1. Preparing data 2. Diversity metrics 3. Analysis of individual OTU frequencies 4. Soil physical and chemical analyses 5. NMDS community analysisen-USThis Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).Bacterial communitiesFungal communitiesHigh-throughput sequencingMetagenomicsR programming environmentNon-metric multidimensional scalingArticle