De Jong, J. A.DeRouchey, Joel M.Tokach, Michael D.Dritz, Steven S.Goodband, Robert D.Woodworth, Jason C.Jones, Cassandra K.Stark, Charles R.2017-11-302017-11-30http://hdl.handle.net/2097/38306Citation: De Jong, J. A., DeRouchey, J. M., Tokach, M. D., Dritz, S. S., Goodband, R. D., Woodworth, J. C., . . . Stark, C. R. (2016). Stability of commercial phytase sources under different environmental conditions. Journal of Animal Science, 94(10), 4259-4266. doi:10.2527/jas2016-0742A 300-d study was conducted to evaluate storage stability of 4 commercially available phytase products under varied environmental conditions. Products used were: 1) Quantum Blue G (AB Vista, Plantation, FL), 2) Ronozyme Hi Phos GT (DSM Nutritional Products, Parsippany, NJ), 3) Axtra Phy TPT (Dupont, Wilmington, DE), and 4) Microtech 5000 Plus (Guangdong Vtr Bio-Tech Co., Ltd., Guangdong, China). Products were stored as pure forms, in a vitamin premix, or a vitamin trace mineral (VTM) premix. Pure products were stored at -20 degrees C, 4 degrees C, 22 degrees C, or 35 degrees C (75% humidity). Vitamin and VTM premixes were stored at 22 degrees C or 35 degrees C (75% humidity). Samples were stored in open-topped paper bags and sampled on d 30, 60, 90, 120, 210, and 300. Stability was determined as the amount of residual phytase activity (% of initial) at each sampling point. For the pure forms, all interactive and main effects of phytase product, time, and storage temperature were significant (P < 0.05). From d 30 to 300, products had similar reductions in phytase activity at the 3 highest temperatures; however, Quantum Blue G, Ronozyme HiPhos GT, and Axtra Phy TPT had reduced (P < 0.05) phytase activity as compared to Microtech 5000 at -20 degrees C. In general, as storage time increased, residual phytase activity decreased (P < 0.05) regardless of product and storage temperature. When product was stored at 4 degrees C and 22 degrees C, phytase activity was greater than that of product stored at -20 degrees C or 35 degrees C, and Microtech 5000 Plus had greater (P < 0.05) stability regardless of time and temperature as compared to the other 3 products. For vitamin and VTM premixes, a time x temperature x product interaction (P < 0.05) was observed. When stored at 22 degrees C, Axtra Phy TPT and Microtech 5000 Plus had reduced residual phytase activity (P < 0.05) when compared to the other 2 products; however, when stored at 35 degrees C Axtra Phy had even further reduced (P < 0.05) activity than the other 3 products regardless of which form the products were stored in. From d 30 to 300 Axtra Phy TPT and Microtech 5000 Plus had the lowest (P < 0.05) residual phytase activity when compared to the others regardless of storage form or temperature. Phytase products stored in VTM premix had decreased (P < 0.05) residual phytase activity when compared to pure products and vitamin premixes. In conclusion, phytase stored for longer than 90 to 120 d, at both high (35 degrees C) and low (-20 degrees C) temperatures when in pure form or as a VTM premix had reduced residual phytase activity.Copyright © 2016. American Society of Animal Science. This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).https://www.animalsciencepublications.org/files/publications/jas/jas-instructions-to-authors-050917.pdfPhytaseStorageStabilityVitamin PremixVitamin Trace MineralPremixArticle