Paneru, GovindThapa, Prem S.McBride, Sean P.Ramm, AdamLaw, Bruce M.Flanders, Bret N.2013-02-132013-02-132012-11-16http://hdl.handle.net/2097/15302Maneuverable, high aspect ratio poly 3-4 ethylene dioxythiophene (PEDOT) fibers are fabricated for use as cellular force probes that can interface with individual pseudopod adhesive contact sites without forming unintentional secondary contacts to the cell. The straight fibers have lengths between 5 and 40 μm and spring constants in the 0.07–23.2 nN μmˉ¹ range. The spring constants of these fibers were measured directly using an atomic force microscope (AFM). These AFM measurements corroborate determinations based on the transverse vibrational resonance frequencies of the fibers, which is a more convenient method. These fibers are employed to characterize the time dependent forces exerted at adhesive contacts between apical pseudopods of highly migratory D. discoideum cells and the PEDOT fibers, finding an average terminal force of 3.1± 2.7 nN and lifetime of 23.4 ± 18.5 s to be associated with these contacts.en-USThis is an author-created, un-copyedited version of an article accepted for publication in Nanotechnology. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The definitive publisher authenticated version is available online at http://iopscience.iop.org/0957-4484/23/45/455105/ .Article (author version)