Three-Dimensional Reconstruction of the Giant Mimivirus Particle with an X-Ray Free-Electron Laser - Ekeberg, T.; Svenda, M.; Abergel, C.; Maia, Frnc; Seltzer, V.; Claverie, J. M.; Hantke, M.; Jonsson, O.; Nettelblad, C.; van der Schot, G.; Liang, M.; DePonte, D. P.; et al. (My University, 2015)
Citation: Ekeberg, T., Svenda, M., Abergel, C., Maia, F., Seltzer, V., Claverie, J. M., . . . Hajdu, J. (2015). Three-Dimensional Reconstruction of the Giant Mimivirus Particle with an X-Ray Free-Electron Laser. Physical Review Letters, 114(9), 6. doi:10.1103/PhysRevLett.114.098102
We present a proof-of-concept three-dimensional reconstruction of the giant mimivirus particle from experimentally measured diffraction patterns from an x-ray free-electron laser. Three-dimensional imaging requires the assembly of many two-dimensional patterns into an internally consistent Fourier volume. Since each particle is randomly oriented when exposed to the x-ray pulse, relative orientations have to be retrieved from the diffraction data alone. We achieve this with a modified version of the expand, maximize and compress algorithm and validate our result using new methods.
Additional Authors: Andersson, I.;Loh, N. D.;Martin, A. V.;Chapman, H.;Bostedt, C.;Bozek, J. D.;Ferguson, K. R.;Krzywinski, J.;Epp, S. W.;Rolles, D.;Rudenko, A.;Hartmann, R.;Kimmel, N.;Hajdu, J.
Keywords: Diffraction Microscopy; Cryomicroscopy; Protein; Pulses; Physics