Pierce, VeronicaKang, MyungshimAburi, MahalaxmiWeerasinghe, SamanthaSmith, Paul E.2011-08-222011-08-222011-07-28http://hdl.handle.net/2097/12054The effect of cosolvents on biomolecular equilibria has traditionally been rationalized using simple binding models. More recently, a renewed interest in the use of Kirkwood–Buff (KB) theory to analyze solution mixtures has provided new information on the effects of osmolytes and denaturants and their interactions with biomolecules. Here we review the status of KB theory as applied to biological systems. In particular, the existing models of denaturation are analyzed in terms of KB theory, and the use of KB theory to interpret computer simulation data for these systems is discussed.This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).Preferential bindingPreferential interactionsKirkwood-Buff theoryProtein denaturationCosolventsOsmolytesComputer simulationArticle (author version)