Mosquera, GloriaGiraldo, Martha C.Khang, Chang HyunCoughlan, SeanValent, Barbara2013-08-092013-08-092009-04-01http://hdl.handle.net/2097/16215Citation: Mosquera, G., . . . Valent, B. (2009). Interaction Transcriptome Analysis Identifies Magnaporthe oryzae BAS1-4 as Biotrophy-Associated Secreted Proteins in Rice Blast Disease. The Plant Cell, 21(4), 1273-1290. https://doi.org/10.1105/tpc.107.055228Biotrophic invasive hyphae (IH) of the blast fungus Magnaporthe oryzae secrete effectors to alter host defenses and cellular processes as they successively invade living rice (Oryza sativa) cells. However, few blast effectors have been identified. Indeed, understanding fungal and rice genes contributing to biotrophic invasion has been difficult because so few plant cells have encountered IH at the earliest infection stages. We developed a robust procedure for isolating infected-rice sheath RNAs in which ∼20% of the RNA originated from IH in first-invaded cells. We analyzed these IH RNAs relative to control mycelial RNAs using M. oryzae oligoarrays. With a 10-fold differential expression threshold, we identified known effector PWL2 and 58 candidate effectors. Four of these candidates were confirmed to be fungal biotrophy-associated secreted (BAS) proteins. Fluorescently labeled BAS proteins were secreted into rice cells in distinct patterns in compatible, but not in incompatible, interactions. BAS1 and BAS2 proteins preferentially accumulated in biotrophic interfacial complexes along with known avirulence effectors, BAS3 showed additional localization near cell wall crossing points, and BAS4 uniformly outlined growing IH. Analysis of the same infected-tissue RNAs with rice oligoarrays identified putative effector-induced rice susceptibility genes, which are highly enriched for sensor-transduction components rather than typically identified defense response genes.en-USPermission to make digital or hard copies of part or all of a work published in Plant Physiology or The Plant Cell is granted without fee for personal or classroom use, provided that copies are not made or distributed for profit or commercial advantage. Please include a citation in any standard format and a notice that the material is copyright by the American Society of Plant Biologists. If the use is commercial or if you wish to make multiple copies other than for educational purposes, please process your request through the Copyright Clearance Center atRice blast diseaseRiceMagnaporthe oryzaeOryza sativaBiotrophic invasive hyphaeText