Blood flow responses to mild-intensity exercise in ectopic versus orthotopic prostate tumors: dependence upon host-tissue hemodynamics and vascular reactivity
dc.contributor.author | Garcia, Emmanuel | |
dc.date.accessioned | 2016-08-04T18:25:20Z | |
dc.date.available | 2016-08-04T18:25:20Z | |
dc.date.graduationmonth | August | en_US |
dc.date.issued | 2016-08-01 | en_US |
dc.date.published | 2016 | en_US |
dc.description.abstract | Given the critical role of tumor O₂ delivery on patient prognosis and the rise in preclinical exercise-oncology studies, we investigated tumor and host-tissue blood flow at rest and during exercise as well as vascular reactivity using a rat prostate cancer model grown in two transplantation sites. Methods. In male COP/CrCrl rats, blood flow (via radiolabeled microspheres) to prostate tumors (R3327-MatLyLu cells injected in the left flank (ectopic) or ventral prostate (orthotopic)) and host-tissue was measured at rest and during a bout of mild-intensity exercise. Alpha-adrenergic vasoconstriction to norepinephrine (NE: 10⁻⁹ to 10⁻⁴ M) was determined in arterioles perforating the tumors and host-tissue. To determine host-tissue exercise hyperemia in healthy tissue, a sham-operated group was included. Results. Blood flow was lower at rest and during exercise in ectopic tumors and host-tissue (subcutaneous adipose) versus the orthotopic tumor and host-tissue (prostate). During exercise, blood flow to the ectopic tumor significantly decreased by 25 ± 5%, whereas flow to the orthotopic tumor increased by 181 ± 30%. Maximal vasoconstriction to NE was not different between arterioles from either tumor location. However, there was a significantly higher peak vasoconstriction to NE in subcutaneous adipose arterioles (92 ± 7%) versus prostate arterioles (55 ± 7%). Establishment of the tumor did not alter host-tissue blood flow from either location at rest or during exercise. Conclusion. These data demonstrate blood flow in tumors is dependent on host-tissue hemodynamics and that the location of the tumor may critically affect how exercise impacts the tumor microenvironment and treatment outcomes. | en_US |
dc.description.advisor | Bradley J. Behnke | en_US |
dc.description.degree | Master of Science | en_US |
dc.description.department | Department of Kinesiology | en_US |
dc.description.level | Masters | en_US |
dc.description.sponsorship | American Cancer Society | en_US |
dc.identifier.uri | http://hdl.handle.net/2097/32858 | |
dc.language.iso | en_US | en_US |
dc.publisher | Kansas State University | en |
dc.subject | Kinesiology | en_US |
dc.subject | Oncology | en_US |
dc.subject | Tumor | en_US |
dc.subject | Hemodynamics | en_US |
dc.title | Blood flow responses to mild-intensity exercise in ectopic versus orthotopic prostate tumors: dependence upon host-tissue hemodynamics and vascular reactivity | en_US |
dc.type | Thesis | en_US |