The Small GTPase MoSec4 Is Involved in Vegetative Development and Pathogenicity by Regulating the Extracellular Protein Secretion in Magnaporthe oryzae

dc.citationZheng, H. K., Chen, S. M., Chen, X. F., Liu, S. Y., Dang, X., Yang, C. D., . . . Valent, B. (2016). The Small GTPase MoSec4 Is Involved in Vegetative Development and Pathogenicity by Regulating the Extracellular Protein Secretion in Magnaporthe oryzae. Frontiers in Plant Science, 7(1), 14. https://doi.org/10.3389/fpls.2016.01458
dc.citation.doi10.3389/fpls.2016.01458
dc.citation.epage27
dc.citation.issn1664-462X
dc.citation.issue1
dc.citation.jtitleFrontiers in Plant Science
dc.citation.spage1
dc.citation.volume7
dc.contributor.authorZheng, H. K.
dc.contributor.authorChen, S. M.
dc.contributor.authorChen, X. F.
dc.contributor.authorLiu, S. Y.
dc.contributor.authorDang, X.
dc.contributor.authorYang, C. D.
dc.contributor.authorGiraldo, M. C.
dc.contributor.authorOliveira-Garcia, E.
dc.contributor.authorZhou, J.
dc.contributor.authorWang, Z. H.
dc.contributor.authorValent, Barbara
dc.contributor.authoreidbvalent
dc.contributor.kstateValent, Barbara
dc.date.accessioned2017-11-30T21:53:41Z
dc.date.available2017-11-30T21:53:41Z
dc.date.issued2016-06-03
dc.date.published2016
dc.descriptionCitation: Zheng, H. K., Chen, S. M., Chen, X. F., Liu, S. Y., Dang, X., Yang, C. D., . . . Valent, B. (2016). The Small GTPase MoSec4 Is Involved in Vegetative Development and Pathogenicity by Regulating the Extracellular Protein Secretion in Magnaporthe oryzae. Frontiers in Plant Science, 7(1), 14. https://doi.org/10.3389/fpls.2016.01458
dc.descriptionArticle: Version of Record
dc.description.abstractThe Rab GTPase proteins play important roles in the membrane trafficking, and consequently protein secretion and development of eukaryotic organisms. However, little is known about the function of Rab GTPases in Magnaporthe oryzae. To further explore the function of Rab GTPases, we deleted the ortholog of the yeast Sec4p protein in M. oryzae, namely MoSEC4. The Delta Mosec4 mutant is defective in polarized growth and conidiation, and it displays decreased appressorium turgor pressure and attenuated pathogenicity. Notably, the biotrophic invasive hyphae produced in rice cells are more bulbous and compressed in the Delta Mosec4 mutant. Further studies showed that deletion of the MoSEC4 gene resulted in decreased secretion of extracellular enzymes and mislocalization of the cytoplasmic effector PWL2-mCherry-NLS. In accordance with a role in secretion, the GFP-MoSec4 fusion protein mainly accumulates at tips of growing vegetative hyphae. Our results suggest that the MoSec4 protein plays important roles in the secretion of extracellular proteins and consequently hyphal development and pathogenicity in the rice blast fungus.
dc.identifier.urihttp://hdl.handle.net/2097/38412
dc.relation.urihttps://doi.org/10.3389/fpls.2016.01458
dc.rightsCopyright © 2016 Dwivedi, Upadhyaya, Chung, De Vita, García-Lara, GuajardoFlores, Gutiérrez-Uribe, Serna-Saldívar, Rajakumar, Sahrawat, Kumar and Ortiz. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectRab Gtpase
dc.subjectExocyst Complex
dc.subjectMosec4
dc.subjectEffector Secretion
dc.subjectPyricularia
dc.subjectOryzae
dc.titleThe Small GTPase MoSec4 Is Involved in Vegetative Development and Pathogenicity by Regulating the Extracellular Protein Secretion in Magnaporthe oryzae
dc.typeText

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